Protein Profiles and Dehydrin Accumulation in Some Soybean Varieties (<i>Glycine max</i>L. Merr) in Drought Stress Conditions  

作　　者：Estri Laras Arumingtyas Evika Sandi Savitri Runik Dyah Purwoningrahayu 

机构地区：[1]Biology Department, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang, Malang, Indonesia [2]Biology Department, Faculty of Science, University of Brawijaya, Malang, Indonesia [3]Research Institute of Legume and Tuber Crops, Malang, Indonesia

出　　处：《American Journal of Plant Sciences》2013年第1期134-141,共8页美国植物学期刊（英文）

摘　　要：Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varieties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.

关 键 词：DEHYDRIN Protein Drought Stress Soybean Varieties LEA 

分 类 号：R73[医药卫生—肿瘤]