Endophytic Mycobiota Characterization of the Amazonian Mistletoe Cladocolea micrantha Hosted in Cashew Tree  

作　　者：Anderson C.Guimaraes Antonio C.Siani JoséL.Bezerra Antonia Q.Lima de Souza Maria Inez M.Sarquis 

机构地区：[1]Instituto de Ciencias Exatas e Tecnologia de Itacoatiara,Universidade Federal do Amazonas,Itacoatiara,Brazil [2]Departamento de Produtos Naturais,Farmanguinhos,Fiocruz,Rio de Janeiro,Brazil [3]Departamento de Ciencias Agronomicas e Ambientais,Universidade Estadual de Santa Cruz,Ilhéus,Brazil [4]Laboratório de Genética,Escola Superior de Ciencias da Saúde,Universidade Estadual do Amazonas,Manaus,Brazil [5]Laboratório de Taxonomia Bioquímica e Bioprospeccao de Fungos,Instituto Oswaldo Cruz,Fiocruz,Rio de Janeiro,Brazil

出　　处：《American Journal of Plant Sciences》2013年第4期917-921,共5页美国植物学期刊（英文）

摘　　要：Endophytic fungi were identified from different parts of the medicinal parasitic mistletoe Cladocolea micrantha and from its host Anacardium occidentale, suggesting a strict host-parasite relationship. Eight fungal endophytes were isolated and morphologically characterized. The ascomycete Guignardia mangiferae and strains of Mycelia sterilia were prevalent in the isolations. The unequivocal identification of Guignardia mangiferae at a probabilistic degree close to 100% was carried out by DNA extraction followed by PCR analyses of the ITS-1 and ITS-2 regions and comparison of the genetic sequence with the NCBI database.Endophytic fungi were identified from different parts of the medicinal parasitic mistletoe Cladocolea micrantha and from its host Anacardium occidentale, suggesting a strict host-parasite relationship. Eight fungal endophytes were isolated and morphologically characterized. The ascomycete Guignardia mangiferae and strains of Mycelia sterilia were prevalent in the isolations. The unequivocal identification of Guignardia mangiferae at a probabilistic degree close to 100% was carried out by DNA extraction followed by PCR analyses of the ITS-1 and ITS-2 regions and comparison of the genetic sequence with the NCBI database.

关 键 词：Cladocolea micrantha Endophytic mycobiota LORANTHACEAE Guignardia mangiferae Anacardium occidentale 

分 类 号：R73[医药卫生—肿瘤]