Morphological and Molecular Dissection of Leaf Development in Wild-Type and Various Morphogenetic Mutants in Rice  

作　　者：Punyavee Dechkrong Takanori Yoshikawa Jun-Ichi Itoh 

机构地区：[1]Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan [2]School of Agricultural Regional Vitalization, Kibi International University, Minamiawaji, Japan

出　　处：《American Journal of Plant Sciences》2015年第8期1215-1232,共18页美国植物学期刊（英文）

摘　　要：Leaf cells undergo two main developmental events;i.e., cell proliferation and cell differentiation, before maturation. These events occur sequentially at specific positions and with specific timing during leaf development. To understand the transition from cell proliferation to cell differentiation of rice leaves, we analyzed rice leaves from both morphological and molecular viewpoints. The results of anatomical, morphological, and histochemical analyses indicated that P4 leaf primordium is the stage when dynamic transition of the cellular state from immature to mature along the proximal-distal axis of the leaf occurs. We screened for marker genes showing a dynamic expression pattern along the proximal-distal axis of the P4 leaf, and applied them to expression analysis in wild-type and various morphogenetic mutants. The changes in expression pattern of the marker genes varied between developmental stages and between mutants. Our analysis reinforced previous data regarding the developmental transition of wild-type rice leaves and indicated that the transition can be monitored using our molecular markers. The results of this study indicate that expression analysis using these molecular markers would be valuable for understanding the genetic effects on leaf development in various leaf morphogenetic mutants.Leaf cells undergo two main developmental events;i.e., cell proliferation and cell differentiation, before maturation. These events occur sequentially at specific positions and with specific timing during leaf development. To understand the transition from cell proliferation to cell differentiation of rice leaves, we analyzed rice leaves from both morphological and molecular viewpoints. The results of anatomical, morphological, and histochemical analyses indicated that P4 leaf primordium is the stage when dynamic transition of the cellular state from immature to mature along the proximal-distal axis of the leaf occurs. We screened for marker genes showing a dynamic expression pattern along the proximal-distal axis of the P4 leaf, and applied them to expression analysis in wild-type and various morphogenetic mutants. The changes in expression pattern of the marker genes varied between developmental stages and between mutants. Our analysis reinforced previous data regarding the developmental transition of wild-type rice leaves and indicated that the transition can be monitored using our molecular markers. The results of this study indicate that expression analysis using these molecular markers would be valuable for understanding the genetic effects on leaf development in various leaf morphogenetic mutants.

关 键 词：RICE Leaf Development CELL Proliferation CELL Differentiation TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF) GRF (GROWTH-REGULATING FACTOR) 

分 类 号：R73[医药卫生—肿瘤]