An Attenuated Strain of Cucumber Green Mottle Mosaic Virus as a Biological Control Agent against Pathogenic Viral Strains  

作　　者：Anna A. Slavokhotova Ekaterina A. Istomina Emma N. Andreeva Tatyana V. Korostyleva Vitalij A. Pukhalskij Alexander N. Shijan Tatyana I. Odintsova Anna A. Slavokhotova;Ekaterina A. Istomina;Emma N. Andreeva;Tatyana V. Korostyleva;Vitalij A. Pukhalskij;Alexander N. Shijan;Tatyana I. Odintsova(Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia)

机构地区：[1]Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

出　　处：《American Journal of Plant Sciences》2016年第5期724-732,共9页美国植物学期刊（英文）

摘　　要：Cucumber green mottle mosaic virus (CGMMV), a member of the Tobamovirus genus, causes a severe disease of cucurbits. In the Moscow region of Russian Federation, the incidence of infection on cucumber plants in greenhouses is high;however, the virus is poorly studied. In this work, the full-length genomes of two pathogenic MC-1 and MC-2 strains of CGMMV isolated from cucumber plants grown in greenhouses in the Moscow region and the attenuated VIROG-43M strain were sequenced. Comparison of VIROG-43M nucleotide sequence with those of the pathogenic strains revealed three missense mutations. Their role in attenuation is discussed. For the first time, in a number of trials conducted under laboratory conditions and in commercial greenhouses, the efficiency of the attenuated VIROG-43M strain as a biocontrol agent for cucumber plant protection resulting in significant yield gain was demonstrated. Phylogenetic analysis with 83 full-length CGMMV coat protein genes isolated in 16 different countries showed that Russian strains are related to isolates from Spain, Greece, USA and Israel.Cucumber green mottle mosaic virus (CGMMV), a member of the Tobamovirus genus, causes a severe disease of cucurbits. In the Moscow region of Russian Federation, the incidence of infection on cucumber plants in greenhouses is high;however, the virus is poorly studied. In this work, the full-length genomes of two pathogenic MC-1 and MC-2 strains of CGMMV isolated from cucumber plants grown in greenhouses in the Moscow region and the attenuated VIROG-43M strain were sequenced. Comparison of VIROG-43M nucleotide sequence with those of the pathogenic strains revealed three missense mutations. Their role in attenuation is discussed. For the first time, in a number of trials conducted under laboratory conditions and in commercial greenhouses, the efficiency of the attenuated VIROG-43M strain as a biocontrol agent for cucumber plant protection resulting in significant yield gain was demonstrated. Phylogenetic analysis with 83 full-length CGMMV coat protein genes isolated in 16 different countries showed that Russian strains are related to isolates from Spain, Greece, USA and Israel.

关 键 词：Cucumber Green Mottle Mosaic Virus TOBAMOVIRUS Attenuated Virus Strain CROSS-PROTECTION Biological Control 

分 类 号：S43[农业科学—农业昆虫与害虫防治]