Plantlets Regeneration via Somatic Embryogenesis from the Nucellus Tissues of Kinnow Mandarin (Citrus reticulata L.)  

作　　者：Mubashir Hussain Naveed Iqbal Raja Muhammad Iqbal Anam Iftikhar Huma Mehreen Sadaf Sidra Sabir Muhammad Asim Sultan Muhammad Nasim Ashraf Faz Mubashir Hussain;Naveed Iqbal Raja;Muhammad Iqbal;Anam Iftikhar;Huma Mehreen Sadaf;Sidra Sabir;Muhammad Asim Sultan;Muhammad Nasim Ashraf Faz(Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan;Department of Botany, University of Sargodha, Sargodha, Pakistan;Department of Botany, Government College University, Faisalabad, Pakistan)

机构地区：[1]Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan [2]Department of Botany, University of Sargodha, Sargodha, Pakistan [3]Department of Botany, Government College University, Faisalabad, Pakistan

出　　处：《American Journal of Plant Sciences》2016年第6期798-805,共8页美国植物学期刊（英文）

摘　　要：Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C₈ medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C₈ medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).

关 键 词：Tissue Culture MS Media Citrus Nucellus Tissue CALLOGENESIS Somatic Embryos 

分 类 号：R73[医药卫生—肿瘤]