Effects of Abscisic Acid Treatment on Berry Coloration and Expression of Flavonoid Biosynthesis Genes in Grape  

作　　者：Ayako Katayama-Ikegami Tomoaki Sakamoto Kana Shibuya Takane Katayama Mei Gao-Takai Ayako Katayama-Ikegami;Tomoaki Sakamoto;Kana Shibuya;Takane Katayama;Mei Gao-Takai(Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Ishikawa, Japan;Graduate School of Biostudies, Kyoto University, Kyoto, Japan)

机构地区：[1]Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Ishikawa, Japan [2]Graduate School of Biostudies, Kyoto University, Kyoto, Japan

出　　处：《American Journal of Plant Sciences》2016年第9期1325-1336,共12页美国植物学期刊（英文）

摘　　要：In order to enhance berry coloration of bright-red grape cultivars, the effects of abscisic acid (ABA) treatment on the quantity and composition of anthocyanins as well as the expression of genes related to flavonoid biosynthesis in the berry were examined. Exogenous ABA treatment increased anthocyanin content, especially petunidin- and malvidin-type anthocyanins. Quantitative real-time PCR analysis revealed that ABA treatment around véraison resulted in the upregulation of genes encoding enzymes responsible for both general flavonoid and anthocyanin biosynthesis. On the other hand, the gene expressions of enzymes involved in proanthocyanidin synthesis were drastically decreased at véraison and remained extremely low even with ABA treatment. Thus, increases in the total amount and composition ratios of petunidin- and malvidin-type anthocyanins were mainly caused by ABA-induced upregulation of uridine diphosphate glucose flavonoid glucosyl transferase, glutathione S-transferase 4, O-methyl transferase and flavonoid 3’, 5’ hydroxylase expression, resulting in the deep coloration of berry of skin.In order to enhance berry coloration of bright-red grape cultivars, the effects of abscisic acid (ABA) treatment on the quantity and composition of anthocyanins as well as the expression of genes related to flavonoid biosynthesis in the berry were examined. Exogenous ABA treatment increased anthocyanin content, especially petunidin- and malvidin-type anthocyanins. Quantitative real-time PCR analysis revealed that ABA treatment around véraison resulted in the upregulation of genes encoding enzymes responsible for both general flavonoid and anthocyanin biosynthesis. On the other hand, the gene expressions of enzymes involved in proanthocyanidin synthesis were drastically decreased at véraison and remained extremely low even with ABA treatment. Thus, increases in the total amount and composition ratios of petunidin- and malvidin-type anthocyanins were mainly caused by ABA-induced upregulation of uridine diphosphate glucose flavonoid glucosyl transferase, glutathione S-transferase 4, O-methyl transferase and flavonoid 3’, 5’ hydroxylase expression, resulting in the deep coloration of berry of skin.

关 键 词：Abscisic Acid (ABA) ANTHOCYANIN Biosynthetic Enzyme Gene Expression Transcription Factor 

分 类 号：R73[医药卫生—肿瘤]