Expression Vector Construction and Genetic Transformation of <i>Rosa rugosa β</i>-l,3-Glucanase Gene (<i>RrGlu</i>)  

作　　者：Shutang Xing Juanjuan Sun Zhihong Peng Yanan Fu Lanyong Zhao Zongda Xu Xiaoyan Yu 

机构地区：[1]Forestry College of Shandong Agricultural University, Tai’an, China [2]College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China [3]Tai’an Landscape Administration Bureau, Tai’an, China

出　　处：《American Journal of Plant Sciences》2017年第3期495-501,共7页美国植物学期刊（英文）

摘　　要：In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.

关 键 词：Rosa rugose β-l 3-GLUCANASE GENE Expression Vector CONSTRUCTION Genetic Transformation 

分 类 号：R73[医药卫生—肿瘤]