Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i>and <i>Raphanus sativus</i>  

作　　者：Soo-Seong Lee Byoung Ho Hwang Tae Yoon Kim Jeongmin Yang Na Rae Han Jongkee Kim Hyun Hee Kim Hadassah Roa Belandres 

机构地区：[1]BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea [2]Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea [3]Plant Biotechnology Institute, Department of Life Science, Sahmyook University, Seoul, Korea

出　　处：《American Journal of Plant Sciences》2017年第6期1345-1356,共12页美国植物学期刊（英文）

摘　　要：A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F1F1) produced by crossing a newly synthesized, unstable allopolyploid (F1) and a stable cultivar, BB#1(F1) in xBrassicoraphanus. An F1F1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F1F1) produced by crossing a newly synthesized, unstable allopolyploid (F1) and a stable cultivar, BB#1(F1) in xBrassicoraphanus. An F1F1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.

关 键 词：xBrassicoraphanus INTERGENERIC Hybrid Baemoochae Brassica rapa RAPHANUS sativus 

分 类 号：R73[医药卫生—肿瘤]