Callogenesis of Cork Oak(Quercus suber L.)through In Vitro Culture of Nodes and Internodes  

作　　者：Mohammed L’bachir El Kbiach Brahim El Bouzdoudi Rabah Saidi Zineb Nejjar El Ansari Safaa Rahmouni Ahmed Lamarti 

机构地区：[1]Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco [2]Department of Matter and Life Sciences, High Normal School, Martil, Morocco

出　　处：《American Journal of Plant Sciences》2017年第8期1801-1819,共19页美国植物学期刊（英文）

摘　　要：The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.

关 键 词：Cork oak Quercus suber L. NODES INTERNODES CALLOGENESIS 

分 类 号：R73[医药卫生—肿瘤]