Development,Validation and Characterization of Genic Microsatellite Markers in Urochloa Species  

作　　者：Juliana Santos Souza Lucimara Chiari Rosangela Maria Simeao Mariane de Mendonca Vilela Leonardo Rippel Salgado 

机构地区：[1]Universidade Anhanguera-Uniderp-Campus Agrárias,Campo Grande,Brazil [2]Embrapa Gado de Corte,Campo Grande,Brazil

出　　处：《American Journal of Plant Sciences》2018年第2期281-295,共15页美国植物学期刊（英文）

基　　金：the Associacao para o Fomentoa Pesquisa de Melhoramento de Sementes Forrageiras(Unipasto),Fundacao de Apoio ao Desenvolvimento do Ensino,Ciencia e Tecnologia do Estado de Mato Grosso do Sul(FUNDECT 0048/12);the National Council for Scientific and Technological Development for financial support.

摘　　要：Urochloa (syn. Brachiaria) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha, U. humidicola and U. ruziziensis. Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens “Basilisk” roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha, U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple Correspondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species.Urochloa (syn. Brachiaria) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha, U. humidicola and U. ruziziensis. Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens “Basilisk” roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha, U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple Correspondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species.

关 键 词：BRACHIARIA Signalgrass Simple Sequence Repeat Transferability Tropical Forage 

分 类 号：R73[医药卫生—肿瘤]