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作 者:Safaa Rahmouni[1] Zineb Nejjar El Ansari[1] Alain Badoc[1] Patrick Martin[1] Mohammed L’Bachir El Kbiach[1] Ahmed Lamarti[1]
出 处:《American Journal of Plant Sciences》2020年第5期626-641,共16页美国植物学期刊(英文)
摘 要:In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.
关 键 词:CORK Oak QUERCUS suber L. Secondary Somatic EMBRYOGENESIS Amino Acids
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