Analysis of Two-Dimensional Gel Electrophoresis Map of Methicillin-Resistant <i>Staphylococcus aureus</i>Treated with Acetone Extract from <i>Canarium odontophyllum</i>Miq. Leaves  

作　　者：Nur Amira Mohd Shamsuddin Dayang Fredalina Basri Noraziah M. Zin Ahmad Raha Raus Nur Faizah Abu Bakar Nur Amira Mohd Shamsuddin;Dayang Fredalina Basri;Noraziah M. Zin;Ahmad Raha Raus;Nur Faizah Abu Bakar(Centre for Diagnostic, Therapeutic & Investigative Studies (CODTIS), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia;Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia)

机构地区：[1]Centre for Diagnostic, Therapeutic & Investigative Studies (CODTIS), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia [2]Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia

出　　处：《American Journal of Plant Sciences》2021年第1期37-52,共16页美国植物学期刊（英文）

摘　　要：Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span><span style="font-family:Verdana;">leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllu</span></i></span><i><span style="font-family:Verdana;">m </span></i><span style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein expMethicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span><span style="font-family:Verdana;">leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllu</span></i></span><i><span style="font-family:Verdana;">m </span></i><span style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein exp

关 键 词：Canarium odontophyllum MRSA 2-DE IEF SDS-PAGE Protein Expression Profile 

分 类 号：R44[医药卫生—诊断学]