DNA Fidelity: Expression of a Monocot Promoter in a Dicot Plant  

作　　者：Luis María Suárez-Rodríguez Hugh Mason Joel Ramírez-Cabrera Luis Jorge Saucedo Miguel Angel Gómez-Lim Charles Arntzen Rodolfo López-Gómez Luis María Suárez-Rodríguez;Hugh Mason;Joel Ramírez-Cabrera;Luis Jorge Saucedo;Miguel Angel Gómez-Lim;Charles Arntzen;Rodolfo López-Gómez(Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México;School of Life Sciences, Arizona State University, Tempe, USA;Departamento de Genética Cinvestav IPN Campus Irapuato, Irapuato, México)

机构地区：[1]Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, México [2]School of Life Sciences, Arizona State University, Tempe, USA [3]Departamento de Genética Cinvestav IPN Campus Irapuato, Irapuato, México

出　　处：《American Journal of Plant Sciences》2022年第1期50-59,共10页美国植物学期刊（英文）

摘　　要：The knowledge generated from the identification of plant promoters has been very important for plant biotechnology development. The use of promoters in transgenic plants allows a reasonable level of regulating protein expression. With the application of reporter genes, such as <i>gus</i>A (<i>uid</i>A,) the production of a colored protein, <i>β</i>-glucuronidase, can be detected and measured both qualitatively and quantitatively, and the activity of the promoter can be assessed. In this work we use a promoter of an abundant banana fruit protein gene <i>Musa acuminata</i> Acidic Chitinase class III a monocot species, to drive expression of <i>gus</i>A in a dicot species, like tomato. We evaluated the monocot promoter capabilities by localizing and quantifying <i>β</i>-glucuronidase (GUS) expression through fluorometric assays during tomato fruit ripening. Our results suggest that this promoter could be used for specifically strong fruit protein expression in dicot plants.The knowledge generated from the identification of plant promoters has been very important for plant biotechnology development. The use of promoters in transgenic plants allows a reasonable level of regulating protein expression. With the application of reporter genes, such as <i>gus</i>A (<i>uid</i>A,) the production of a colored protein, <i>β</i>-glucuronidase, can be detected and measured both qualitatively and quantitatively, and the activity of the promoter can be assessed. In this work we use a promoter of an abundant banana fruit protein gene <i>Musa acuminata</i> Acidic Chitinase class III a monocot species, to drive expression of <i>gus</i>A in a dicot species, like tomato. We evaluated the monocot promoter capabilities by localizing and quantifying <i>β</i>-glucuronidase (GUS) expression through fluorometric assays during tomato fruit ripening. Our results suggest that this promoter could be used for specifically strong fruit protein expression in dicot plants.

关 键 词：PROMOTER Fruit RIPENING Tomato BANANA 

分 类 号：Q94[生物学—植物学]