Protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea  

作　　者：Mounyratou Rabo Teyioue Benoit Joseph Batieno Assita Traoré-Barro Salimata Traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar Traoré Mounyratou Rabo;Teyioue Benoit Joseph Batieno;Assita Traoré-Barro;Salimata Traoré;Orokia Coulibaly;Aboubacar Toguyeni;Chantal Kaboré-Zoungrana;Oumar Traoré(Laboratoire National de Biosécurité, Agence Nationale de Biosécurité (ANB), Ouagadougou, Burkina Faso;Institut de l’Environnement et Recherches Agricoles, Département Productions Végétales, Ouagadougou, Burkina Faso;Laboratoire d’Etudes et de Recherches sur les Ressources Naturelles et les Sciences de l’Environnement (LERNSE), Université Nazi BONI, Bobo-Dioulasso, Burkina Faso;Laboratoire de Génétique et amélioration des plantes (LAGAMEP), Institut de l’Environnement et Recherches Agricoles, Ouagadougou, Burkina Faso;Laboratoire de protection et défense des cultures (LAPRODEC), Institut de l’Environnement et Recherches Agricoles, Ouagadougou, Burkina Faso)

机构地区：[1]Laboratoire National de Biosécurité, Agence Nationale de Biosécurité (ANB), Ouagadougou, Burkina Faso [2]Institut de l’Environnement et Recherches Agricoles, Département Productions Végétales, Ouagadougou, Burkina Faso [3]Laboratoire d’Etudes et de Recherches sur les Ressources Naturelles et les Sciences de l’Environnement (LERNSE), Université Nazi BONI, Bobo-Dioulasso, Burkina Faso [4]Laboratoire de Génétique et amélioration des plantes (LAGAMEP), Institut de l’Environnement et Recherches Agricoles, Ouagadougou, Burkina Faso [5]Laboratoire de protection et défense des cultures (LAPRODEC), Institut de l’Environnement et Recherches Agricoles, Ouagadougou, Burkina Faso

出　　处：《American Journal of Plant Sciences》2023年第12期1453-1463,共11页美国植物学期刊(英文)

摘　　要：The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.

关 键 词：COWPEA Protein CRY1AB Protein Fractions 

分 类 号：R73[医药卫生—肿瘤]