GC-MS Analysis and in vivo Anti-Inflammatory, Analgesic Activities of Phyllanthus niruri Linn and Sida acuta Burm, Used to Treat Malaria in Togo  

作　　者：Sossawè Poli Ablam Alognon Kodjo Eloh Luckman Gbati Essoham Ataba Efui Holaly Gbekley Gerard Toudji Bouraïma Djeri Damintoti Simplice Karou Sossawè Poli;Ablam Alognon;Kodjo Eloh;Luckman Gbati;Essoham Ataba;Efui Holaly Gbekley;Gerard Toudji;Bouraïma Djeri;Damintoti Simplice Karou(Laboratoire de Microbiologie et de Contrle de Qualit des Denres Alimentaires, Ecole Suprieure des Techniques Biologiques et Alimentaires, Universit de Lom, Lom, Togo;Laboratoire de Chimie Organique et des Sciences Environnementales, Dpartement de Chimie, Universit de Kara, Kara, Togo;Programme National de Lutte contre le Paludisme, Ministre de la Sant et de lHygine Publique, Lom, Togo)

机构地区：[1]Laboratoire de Microbiologie et de Contrle de Qualit des Denres Alimentaires, Ecole Suprieure des Techniques Biologiques et Alimentaires, Universit de Lom, Lom, Togo [2]Laboratoire de Chimie Organique et des Sciences Environnementales, Dpartement de Chimie, Universit de Kara, Kara, Togo [3]Programme National de Lutte contre le Paludisme, Ministre de la Sant et de lHygine Publique, Lom, Togo

出　　处：《American Journal of Plant Sciences》2024年第12期1162-1184,共23页美国植物学期刊(英文)

摘　　要：The use of plants in traditional medicine is an alternative solution to address the adverse effects of conventional anti-inflammatories. This study evaluated the phytochemistry, anti-inflammatory, analgesic and toxicity activities of the hydroethanolic extracts (HE) alone and the combination of Phyllanthus niruri Linn and Sida acuta Burm. Total phenolics were quantified using the Folin-Ciocalteu method, condensed tannin by the Butanol-HCl method, and flavonoid by the Aluminum chloride method. Gas chromatography coupled with Mass Spectrometry (GC-MS) analysis was used to identify several compounds in the extracts. Anti-inflammatory activity and analgesic activity, respectively, were carried out using the paw edema method in rats and pain method induced by intraperitoneal injection of acetic acid in the animal model. Acute, and subacute oral toxicity of extracts was conducted according to OECD n˚423 for the testing of chemicals and n˚407 for testing chemical products. Screening of S. acuta and P. niruri revealed that both of them contain alkaloids, phenolic compounds, terpenes and flavonoids, but P. niruri is richer in total phenols 14.69 ± 0.22 mg GAE/g;proanthocyanidins 4.42 ± 0.01 mg CE/g;and flavonoids 264.21 ± 3.64 mg RE/g. GC-MS analysis was presented for S. acuta 13 and P. niruri 19 compounds. The compounds most represented are levoglucosan (16.01%) and neophytadiene (10.48%) for S. acuta and 2-[(3,4-Dimethoxyphenyl) methyl]-4-methoxy-4-oxobutanoicacid (24%), and (3R,4R)-3-(Benzo[d][1,3] dioxol-5-ylmethyl)-4-(3,4 dimethoxybenzyl) dihydrofuran-2(3H)-one (18.34%) for P. niruri. The concentration of 400 mg/kg recorded the best anti-inflammatory activity. The analgesic effect’s results showed that the hydroethanolic compound at a concentration of 800 mg/kg recorded the highest percentage inhibition (71.60% ± 5.32%) by the oral route. Toxicity results showed that the extracts alone and in combination also did not exhibit signs of toxicity. These results therefore validate the use of S. acuta and P. niruriThe use of plants in traditional medicine is an alternative solution to address the adverse effects of conventional anti-inflammatories. This study evaluated the phytochemistry, anti-inflammatory, analgesic and toxicity activities of the hydroethanolic extracts (HE) alone and the combination of Phyllanthus niruri Linn and Sida acuta Burm. Total phenolics were quantified using the Folin-Ciocalteu method, condensed tannin by the Butanol-HCl method, and flavonoid by the Aluminum chloride method. Gas chromatography coupled with Mass Spectrometry (GC-MS) analysis was used to identify several compounds in the extracts. Anti-inflammatory activity and analgesic activity, respectively, were carried out using the paw edema method in rats and pain method induced by intraperitoneal injection of acetic acid in the animal model. Acute, and subacute oral toxicity of extracts was conducted according to OECD n˚423 for the testing of chemicals and n˚407 for testing chemical products. Screening of S. acuta and P. niruri revealed that both of them contain alkaloids, phenolic compounds, terpenes and flavonoids, but P. niruri is richer in total phenols 14.69 ± 0.22 mg GAE/g;proanthocyanidins 4.42 ± 0.01 mg CE/g;and flavonoids 264.21 ± 3.64 mg RE/g. GC-MS analysis was presented for S. acuta 13 and P. niruri 19 compounds. The compounds most represented are levoglucosan (16.01%) and neophytadiene (10.48%) for S. acuta and 2-[(3,4-Dimethoxyphenyl) methyl]-4-methoxy-4-oxobutanoicacid (24%), and (3R,4R)-3-(Benzo[d][1,3] dioxol-5-ylmethyl)-4-(3,4 dimethoxybenzyl) dihydrofuran-2(3H)-one (18.34%) for P. niruri. The concentration of 400 mg/kg recorded the best anti-inflammatory activity. The analgesic effect’s results showed that the hydroethanolic compound at a concentration of 800 mg/kg recorded the highest percentage inhibition (71.60% ± 5.32%) by the oral route. Toxicity results showed that the extracts alone and in combination also did not exhibit signs of toxicity. These results therefore validate the use of S. acuta and P. niruri

关 键 词：Sida acuta Phyllanthus niruri PHYTOCHEMISTRY ANTI-INFLAMMATORY ANALGESIC Toxicity TOGO 

分 类 号：R28[医药卫生—中药学]