Regulation of Hematopoietic Activity Involving New Interacting Partners (RRAGC &PSMC2, CKAP4 &MANF and CTR9 &CNTNAP2)  

作　　者：Swati Sharma Gurudutta U. Gangenahalli Upma Singh Swati Sharma;Gurudutta U. Gangenahalli;Upma Singh(Department of Pharmacology, All India Institute of Medical Science (AIIMS), New Delhi, India;Stem Cell & Gene Therapy Research Group, Institute of Nuclear Medicine & Allied Sciences, New Delhi, India;Department of Applied Chemistry, School of Vocational Studies & Applied Sciences, Gautam Buddha University, Greater Noida, India)

机构地区：[1]Department of Pharmacology, All India Institute of Medical Science (AIIMS), New Delhi, India [2]Stem Cell & Gene Therapy Research Group, Institute of Nuclear Medicine & Allied Sciences, New Delhi, India [3]Department of Applied Chemistry, School of Vocational Studies & Applied Sciences, Gautam Buddha University, Greater Noida, India

出　　处：《CellBio》2020年第3期123-141,共19页细胞生物学（英文）

摘　　要：Hematopoietic stem cells (HSCs) are tissue-specific cells giving rise to all mature blood cell types regulated by a diverse group of hematopoietic cytokines and growth factors that influences the survival & proliferation of early progenitors and differentiation mechanisms by modulating the functional activities of HSCs. In this study, the functional yet distinctive role of three novel combinations of gene pairs RRAGC & PSMC2;CKAP4 & MANF;and CTR9 & CNTNAP2 have been newly identified. These novel combinations of genes were confirmed and expressed in K562 human leukemic cell line in the presence of cytokine combination (IL-3, FLT-3 and SCF) using RT-PCR and siRNA-mediated gene knock down strategy. This study signifies the synergistic role of gene pairs in different molecular activities like ubiquitination or proteasomal degradation, calcium mobilization, dopamine signaling.Hematopoietic stem cells (HSCs) are tissue-specific cells giving rise to all mature blood cell types regulated by a diverse group of hematopoietic cytokines and growth factors that influences the survival & proliferation of early progenitors and differentiation mechanisms by modulating the functional activities of HSCs. In this study, the functional yet distinctive role of three novel combinations of gene pairs RRAGC & PSMC2;CKAP4 & MANF;and CTR9 & CNTNAP2 have been newly identified. These novel combinations of genes were confirmed and expressed in K562 human leukemic cell line in the presence of cytokine combination (IL-3, FLT-3 and SCF) using RT-PCR and siRNA-mediated gene knock down strategy. This study signifies the synergistic role of gene pairs in different molecular activities like ubiquitination or proteasomal degradation, calcium mobilization, dopamine signaling.

关 键 词：Hematopoietic Stem Cells (HSCs) Signal Transduction HAEMATOPOIESIS 

分 类 号：R73[医药卫生—肿瘤]