Structural and Functional Annotation of Hypothetical Protein of Fusobacterium nucleatum Strain MJR7757B: An in Silico Approach  

作　　者：Md. Isrfil Hossen Fouzia Mostafa Nusrat Jahan Jannatul Ferdaus Amgad Albahi Sayed Mashequl Bari Md. Isrfil Hossen;Fouzia Mostafa;Nusrat Jahan;Jannatul Ferdaus;Amgad Albahi;Sayed Mashequl Bari(College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China;Department of Crop Science and Technology, Rajshahi University, Rajshahi, Bangladesh;Abdul Malek Ukil Medical College, Noakhali, Bangladesh;Department of Medicine, IBN Sina Medical College, Dhaka, Bangladesh;National Food Research Centre, Khartoum, Sudan;Department of Aquatic Animal Health Management, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh)

机构地区：[1]College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China [2]Department of Crop Science and Technology, Rajshahi University, Rajshahi, Bangladesh [3]Abdul Malek Ukil Medical College, Noakhali, Bangladesh [4]Department of Medicine, IBN Sina Medical College, Dhaka, Bangladesh [5]National Food Research Centre, Khartoum, Sudan [6]Department of Aquatic Animal Health Management, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

出　　处：《Computational Molecular Bioscience》2024年第1期17-33,共17页计算分子生物学（英文）

摘　　要：Fusobacterium nucleatum is an anaerobic, commensal, gram-negative oral bacterium that is carcinogenic and causes a wide range of human diseases. The present study focused on the analysis of the hypothetical protein, HMPREF3221_01179, derived from F. nucleatum strain MJR7757B, employing various computational methods to anticipate both its structure and functional characteristics. NCBI conserved domain analysis, NCBI BLASTp and MEGA Phylogenetic tree study characterize the target protein as an outer membrane efflux protein (ToIC family) which facilitate the bacterial transmembrane transport. With a molecular weight of 52120.02 Da, an isoelectric point (pI) of 8.33, and an instability index of 29.47, the protein is anticipated to exhibit good solubility in the extracellular space and crucial stability for pharmaceutical applications. The protein’s structure meets quality standards during the construction and refinement of its 3D model. The efflux inhibitor Arginine beta-naphthylamide exhibits a significant binding affinity (-7.1 kcal/mol) to the binding site of the target protein. The in-silico analysis improves the understanding of the protein and facilitates future investigations into therapeutic medication.Fusobacterium nucleatum is an anaerobic, commensal, gram-negative oral bacterium that is carcinogenic and causes a wide range of human diseases. The present study focused on the analysis of the hypothetical protein, HMPREF3221_01179, derived from F. nucleatum strain MJR7757B, employing various computational methods to anticipate both its structure and functional characteristics. NCBI conserved domain analysis, NCBI BLASTp and MEGA Phylogenetic tree study characterize the target protein as an outer membrane efflux protein (ToIC family) which facilitate the bacterial transmembrane transport. With a molecular weight of 52120.02 Da, an isoelectric point (pI) of 8.33, and an instability index of 29.47, the protein is anticipated to exhibit good solubility in the extracellular space and crucial stability for pharmaceutical applications. The protein’s structure meets quality standards during the construction and refinement of its 3D model. The efflux inhibitor Arginine beta-naphthylamide exhibits a significant binding affinity (-7.1 kcal/mol) to the binding site of the target protein. The in-silico analysis improves the understanding of the protein and facilitates future investigations into therapeutic medication.

关 键 词：Fusobacterium nucleatum In Silico BACTERIA Hypothetical Protein Molecular Docking 

分 类 号：R73[医药卫生—肿瘤]