Vitamins C, E, and NADH on in Vitro Lymphocyte Proliferation and Redox Status among Obese Patients  

作　　者：Asmaa Meraou Hafida Merzouk Amel Saidi Amel Medjdoub Sid Ahmed Merzouk Slimane Belbraouet Asmaa Meraou;Hafida Merzouk;Amel Saidi;Amel Medjdoub;Sid Ahmed Merzouk;Slimane Belbraouet(Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaid of Tlemcen, Tlemcen, Algeria;University Djilali liabes of Sidi Bel Abbes, Sidi Bel Abbes, Algeria;Department of Technical Sciences, Faculty of Engineering, University Abou-Bekr Belkaid of Tlemcen, Tlemcen, Algeria;Ecole de Nutrition, Université de Moncton, Moncton, Canada)

机构地区：[1]Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaid of Tlemcen, Tlemcen, Algeria [2]University Djilali liabes of Sidi Bel Abbes, Sidi Bel Abbes, Algeria [3]Department of Technical Sciences, Faculty of Engineering, University Abou-Bekr Belkaid of Tlemcen, Tlemcen, Algeria [4]Ecole de Nutrition, Université de Moncton, Moncton, Canada

出　　处：《Food and Nutrition Sciences》2016年第12期1082-1098,共17页食品与营养科学（英文）

摘　　要：Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.

关 键 词：OBESITY LYMPHOCYTES VITAMINS CYTOKINES Oxidant/Antioxidant Status 

分 类 号：O62[理学—有机化学]