机构地区：[1]BASF Corporation, Research Triangle Park, NC, USA
出 处：《Food and Nutrition Sciences》2021年第11期1041-1061,共21页食品与营养科学（英文）
摘 要：RF3 <i>Brassica napus</i> L. (InVigor<sup>TM</sup> Canola) has been in commercial cultivation <span>since 1997. RF3 <i>B. napus</i> was produced by <i>Agrobacterium</i>-mediated tran</span>sformation to provide glufosniate ammonium herbicide tolerance to the crop. Canola quality (CQ) varieties of <i>Brassica juncea</i> L. (Czern & Coss), a close relative of <i>B. napus</i>, have been available in Canada for more than 20 years. Currently, BASF Agricultural Solutions is developing CQ RF3 <i>B. juncea</i> via conventional breeding with RF3<i> B. napus </i>to provide similar herbicide tolerance in the <i>B.</i> <i>juncea</i> species. The insertion event RF3<i> </i>contains the <i>bar</i> gene <span>(origin <i>Streptomyces hygroscopicus</i>) coding for phosphinothricin acetyl trans</span>ferase (PAT/<i>bar</i>) protein which confers tolerance to glufosinate-ammonium<span> </span>herbicides. RF3 also contains the <i>barstar</i> gene (origin <i>Bacillus amyloliquefa<span>ciens</span></i><span>), coding for the Barstar protein, which is an inhibitor of the Barna</span>se protein. In the absence of Barnase, there is no impact of Barstar. Many safety <span>studies have been conducted during the development of the canola quality lines of RF3 </span><i><span>B. napus</span></i><span> and RF3 </span><i><span>B. juncea</span></i><span>. This report seeks to compare th</span>e <span>two species based on the scientific data generated during the safety asses</span>sments of RF3 <i>B. napus</i> and RF3 <i>B. juncea</i> and discusses the utility of using studies with one species to support the safety of the other species both containing the same insertion event.RF3 <i>Brassica napus</i> L. (InVigor<sup>TM</sup> Canola) has been in commercial cultivation <span>since 1997. RF3 <i>B. napus</i> was produced by <i>Agrobacterium</i>-mediated tran</span>sformation to provide glufosniate ammonium herbicide tolerance to the crop. Canola quality (CQ) varieties of <i>Brassica juncea</i> L. (Czern & Coss), a close relative of <i>B. napus</i>, have been available in Canada for more than 20 years. Currently, BASF Agricultural Solutions is developing CQ RF3 <i>B. juncea</i> via conventional breeding with RF3<i> B. napus </i>to provide similar herbicide tolerance in the <i>B.</i> <i>juncea</i> species. The insertion event RF3<i> </i>contains the <i>bar</i> gene <span>(origin <i>Streptomyces hygroscopicus</i>) coding for phosphinothricin acetyl trans</span>ferase (PAT/<i>bar</i>) protein which confers tolerance to glufosinate-ammonium<span> </span>herbicides. RF3 also contains the <i>barstar</i> gene (origin <i>Bacillus amyloliquefa<span>ciens</span></i><span>), coding for the Barstar protein, which is an inhibitor of the Barna</span>se protein. In the absence of Barnase, there is no impact of Barstar. Many safety <span>studies have been conducted during the development of the canola quality lines of RF3 </span><i><span>B. napus</span></i><span> and RF3 </span><i><span>B. juncea</span></i><span>. This report seeks to compare th</span>e <span>two species based on the scientific data generated during the safety asses</span>sments of RF3 <i>B. napus</i> and RF3 <i>B. juncea</i> and discusses the utility of using studies with one species to support the safety of the other species both containing the same insertion event.
关 键 词：CANOLA brassica JUNCEA NAPUS RF3 Composition Protein Expression Molecular Characterization
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