The Influence of Caldesmon Suppression on Proliferation and Motilities of Vascular Smooth Muscle Cells  

作　　者：Bin Gao Qifeng Jiang Bin Gao;Qifeng Jiang(Department of Medical Information, Chongqing Medical University, Chongqing, China;Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing, China)

机构地区：[1]Department of Medical Information, Chongqing Medical University, Chongqing, China [2]Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing, China

出　　处：《Journal of Biomedical Science and Engineering》2016年第9期430-436,共7页生物医学工程（英文）

摘　　要：Migration of vascular smooth muscle cells (VSMCs) from the media to intima constitutes a critical step in the development of proliferative vascular diseases. To elucidate the regulatory mechanism of VSMCs motility, the roles of caldesmon (CaD) were investigated previously. CaD is an actin-binding protein dynamically regulating cytoskeleton structure. In this study, the potential role of CaD in mediating proliferation and motility of VSMCs were discussed. First, structural effect of CaD on cytoskeleton integrity was analyzed with CaD knock-down;second, the proliferation of VSMCs was measured in CaD knock-down and control cells;third, the specific role of CaD on VSMCs motilities was evaluated with in vitro migration and invasion assays. We found that CaD is an integral component to maintain cytoskeleton integrity of VSMCs. Our data indicated that CaD suppression does not show significant influence on VSMCs proliferation, but negatively modulates the motilities of VSMCs, and CaD depletion would significantly facilitate migration and invasion of VSMCs.Migration of vascular smooth muscle cells (VSMCs) from the media to intima constitutes a critical step in the development of proliferative vascular diseases. To elucidate the regulatory mechanism of VSMCs motility, the roles of caldesmon (CaD) were investigated previously. CaD is an actin-binding protein dynamically regulating cytoskeleton structure. In this study, the potential role of CaD in mediating proliferation and motility of VSMCs were discussed. First, structural effect of CaD on cytoskeleton integrity was analyzed with CaD knock-down;second, the proliferation of VSMCs was measured in CaD knock-down and control cells;third, the specific role of CaD on VSMCs motilities was evaluated with in vitro migration and invasion assays. We found that CaD is an integral component to maintain cytoskeleton integrity of VSMCs. Our data indicated that CaD suppression does not show significant influence on VSMCs proliferation, but negatively modulates the motilities of VSMCs, and CaD depletion would significantly facilitate migration and invasion of VSMCs.

关 键 词：CALDESMON PROLIFERATION MOTILITY VSMCS 

分 类 号：R54[医药卫生—心血管疾病]