Single Layer Centrifugation with Androcoll-E^(TM) improved progressive motility and percentage of live spermatozoa with intact acrosome of chilled stallion semen but did not have an effect on DNA integrity  

作　　者：Ana Luisa Costa Ana Martins-Bessa Ana Rebello de Andrade Tiago Guimaraes Maria Rosa Rebordao Sandra Gamboa Pedro Pinto Bravo Maria José Correia Jorge Colaco Isabel Gaivao António Rocha 

机构地区：[1]Centro de Ciencia Animal e Veterinária(CECAV),University of Trás-os-Montes e Alto Douro,Quinta de Prados,Vila Real,Portugal [2]Veterinary Degree,Universty of Tras-os-Montes e Alto Douro,Quinta de Prados,Vila Real,Portugal [3]Centro de Estudos em Ciencia Animal(CECA),Instituto de Ciencias Biomedicas Abel Salazar(ICBAS),University of Porto,Oporto,Portugal [4]Coimbra Higher School of Agriculture,Sao Martinho do Bispo,Coimbra,Portugal [5]Alter Real Foundation,Alter do Chao,Portugal

出　　处：《Open Journal of Animal Sciences》2012年第3期159-165,共7页动物科学期刊（英文）

基　　金：partially financed by Projects PTDC/CVT/108456/2008(FCT)and COMPETE:FCOMP-01-0124-FEDER-009565(FEDER),“Development of methods to increase the fertilizing ability of chilled and frozen stallion semen:a multidisciplinary ap-proach”.

摘　　要：A significant number of stallions produce low quality ejaculates with high sensibility to chilling. Single Layer Centrifugation (SLC) with Andro-coll-ETM has been presented as an efficient method of selecting good quality spermatozoa. In the current study, changes in sperm quality (motility, viability, acrosome integrity and DNA damage) occurring during storage at 5?C for a maximum of 72 h, were investigated. For that, one ejaculate from 12 stallions was split in two aliquots: control and SLC-selected. Both aliquots were chilled and stored at 5?C and spermatozoa were evaluated for motility, viability and acrosome integrity at 24, 48 and 72 h post collection. DNA damage was evaluated at 48 h post collection using the comet assay. In the SLC-selected aliquots, there was a significant improvement in terms of progressive motility (0 h: P = 0.005;24 h: P 0.05). SLC with Androcoll-ETM improved semen quality prolonging sperm longevity of chilled semen (P = 0.012). This positive effect was more evident in ejaculates most sensitive to chilling that had a sharp decrease in motility in the first 24 h of refrigeration and for all ejaculates at 72 h post-chilling. Therefore, this method reveals to be a useful technique for selecting spermatozoa and maintain sperm quality during storage.A significant number of stallions produce low quality ejaculates with high sensibility to chilling. Single Layer Centrifugation (SLC) with Andro-coll-ETM has been presented as an efficient method of selecting good quality spermatozoa. In the current study, changes in sperm quality (motility, viability, acrosome integrity and DNA damage) occurring during storage at 5?C for a maximum of 72 h, were investigated. For that, one ejaculate from 12 stallions was split in two aliquots: control and SLC-selected. Both aliquots were chilled and stored at 5?C and spermatozoa were evaluated for motility, viability and acrosome integrity at 24, 48 and 72 h post collection. DNA damage was evaluated at 48 h post collection using the comet assay. In the SLC-selected aliquots, there was a significant improvement in terms of progressive motility (0 h: P = 0.005;24 h: P 0.05). SLC with Androcoll-ETM improved semen quality prolonging sperm longevity of chilled semen (P = 0.012). This positive effect was more evident in ejaculates most sensitive to chilling that had a sharp decrease in motility in the first 24 h of refrigeration and for all ejaculates at 72 h post-chilling. Therefore, this method reveals to be a useful technique for selecting spermatozoa and maintain sperm quality during storage.

关 键 词：Stallion Sperm Single Layer Centrifugation Androcoll-ETM Membrane Integrity Comet assay Component Formatting Style Styling 

分 类 号：R73[医药卫生—肿瘤]