Sex Determination in the Near Threatened Guadalupe Fur Seal: Molecular Markers and Their Potential Applications  

作　　者：Simona Sanvito Anna Fabiani Filippo Galimberti 

机构地区：[1]Elephant Seal Research Group, Sea Lion Island, Falkland Islands [2]Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Ensenada, México [3]Dipartimento di Biologia, Università Degli Studi di Roma Tor Vergata, Roma, Italia

出　　处：《Open Journal of Animal Sciences》2014年第5期270-277,共8页动物科学期刊（英文）

摘　　要：The determination of sex by simple observation is often difficult in marine mammals, even in sexually dimorphic species. Moreover, there is often the practical necessity to determine sex in samples that have been collected without handling or observing the subjects. In these cases, a molecular assay for sex determination is required. We adapted an assay that targets the zinc-finger region and is based on a single primer pair, to the Guadalupe fur seal, a near threatened species that has a very limited breeding range. First, we validated the assay with a sample of pups in which we determined the sex by direct observation during handling;second, we compared it to a more recent assay, based on two different primer pairs and, finally, we evaluated the effect of DNA quantity on its reliability. The assay that we tested produced excellent results and was more reliable than the other one based on two primers. Reliable results were also obtained when only remarkably small quantities of DNA were amplified. These results show the potential use of this molecular assay in case of non-invasive sampling, an overly common situation when dealing with species of problematic conservation status.The determination of sex by simple observation is often difficult in marine mammals, even in sexually dimorphic species. Moreover, there is often the practical necessity to determine sex in samples that have been collected without handling or observing the subjects. In these cases, a molecular assay for sex determination is required. We adapted an assay that targets the zinc-finger region and is based on a single primer pair, to the Guadalupe fur seal, a near threatened species that has a very limited breeding range. First, we validated the assay with a sample of pups in which we determined the sex by direct observation during handling;second, we compared it to a more recent assay, based on two different primer pairs and, finally, we evaluated the effect of DNA quantity on its reliability. The assay that we tested produced excellent results and was more reliable than the other one based on two primers. Reliable results were also obtained when only remarkably small quantities of DNA were amplified. These results show the potential use of this molecular assay in case of non-invasive sampling, an overly common situation when dealing with species of problematic conservation status.

关 键 词：SEX Determination Sex-Linked Genetic Markers Low-Invasive Sampling Non-Invasive Sampling PINNIPEDS ARCTOCEPHALUS townsendi 

分 类 号：R73[医药卫生—肿瘤]