The Role of CD4+ and CD8+ T-Cells during Angiostrongylus vasorum Infection in Dogs  

作　　者：Thales Augusto Barcante Lis Ribeiro do Valle Antonelli Kenneth Gollob Thiago Pasqua Narciso Silvio Divino de Oliveira Junior Vitor Marcio Ribeiro Ricardo Toshio Fujiwara Ana Paula Peconick Debora Negrao-Correa Walter dos Santos Lima Joziana Muniz de Paiva Barcante 

机构地区：[1]Laboratorio de Biologia Parasitaria(BIOPAR),Departamento de Medicina Veterinaria,Setor Preventiva,Universidade Federal de Lavras(UFLA),Lavras,Brazil [2]Instituto de Ciencias Biologicas,Universidade Federal de Minas Gerais,Belo Horizonte,Brazil [3]Programa de Pos-graduacao em Ciencias Veterinarias,Departamento de Medicina Veterinaria,Universidade Federal de Lavras(UFLA),Lavras,Brazil

出　　处：《Open Journal of Animal Sciences》2014年第5期285-290,共6页动物科学期刊（英文）

基　　金：partially supported by FAPEMIG and CNPq—Brazil.

摘　　要：A determinant factor in the pathology of canine angiostrongylosis seems to be related to the location of the parasite in the definitive host. Their presence inside the arteries and its branches, promoting mechanical and metabolic action on the wall of the vessels, may alter its homeostasis. Bronchoalveolar lavage (BAL) is a procedure that retrieves cells and other elements from de lungs for evaluation, and helps in the diagnosis of many pulmonary diseases. The aim of this study was to evaluate CD4+/CD8+ lymphocyte profile during the infection by Angiostrongylus vasorum, using cells retrieved using BAL. The identification of subpopulations of T lymphocytes by evaluating the co-expression of CD4 and CD8 receptor proteins has shown that despite the increase in both populations, there was a predominance of CD4+ T-cells, instead of CD8+ T-cells. These increases of CD4+ T-cells associated with the increase of the ratio between CD4+/CD8+ suggest polarization of a Th2 response. However, the immune cells, signaling factors, and cytokines that mediate such immunity and how and where they act within the body remain largely undefined during angiostrongylosis.A determinant factor in the pathology of canine angiostrongylosis seems to be related to the location of the parasite in the definitive host. Their presence inside the arteries and its branches, promoting mechanical and metabolic action on the wall of the vessels, may alter its homeostasis. Bronchoalveolar lavage (BAL) is a procedure that retrieves cells and other elements from de lungs for evaluation, and helps in the diagnosis of many pulmonary diseases. The aim of this study was to evaluate CD4+/CD8+ lymphocyte profile during the infection by Angiostrongylus vasorum, using cells retrieved using BAL. The identification of subpopulations of T lymphocytes by evaluating the co-expression of CD4 and CD8 receptor proteins has shown that despite the increase in both populations, there was a predominance of CD4+ T-cells, instead of CD8+ T-cells. These increases of CD4+ T-cells associated with the increase of the ratio between CD4+/CD8+ suggest polarization of a Th2 response. However, the immune cells, signaling factors, and cytokines that mediate such immunity and how and where they act within the body remain largely undefined during angiostrongylosis.

关 键 词：Citometria ANGIOSTRONGYLIASIS Brochoalveolar Lavage 

分 类 号：R73[医药卫生—肿瘤]