Effects of Serum Concentration, Synchronization Time and Confluence on the Cell-Cycle Synchronization Efficiency of Goat Fibroblasts  

作　　者：Van Khanh Nguyen Huong Thu Thi Vu Au Thi Hoang Yen Kim Thi Pham Giang Thi Thanh Nhan Hung Phu Lai Lan Doan Pham Van Khanh Nguyen;Huong Thu Thi Vu;Au Thi Hoang;Yen Kim Thi Pham;Giang Thi Thanh Nhan;Hung Phu Lai;Lan Doan Pham(Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Science, Hanoi, Vietnam)

机构地区：[1]Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Science, Hanoi, Vietnam

出　　处：《Open Journal of Animal Sciences》2024年第3期194-203,共10页动物科学期刊（英文）

摘　　要：This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency.This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency.

关 键 词：Goat Fibroblast Cycle Synchronisation Serum Concentration Synchronization Time CONFLUENCE 

分 类 号：R73[医药卫生—肿瘤]