Transcriptome analysis of the <i>Tityus serrulatus</i>scorpion venom gland  

作　　者：Erika R. Alvarenga Thaís M. Mendes Bárbara F. Magalhaes Flávia F. Siqueira Arthur E. Dantas Tatiana M. Barroca Carolina C. Horta Evanguedes Kalapothakis 

机构地区：[1]Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

出　　处：《Open Journal of Genetics》2012年第4期210-220,共11页遗传学期刊（英文）

摘　　要：The Tityus serrulatus scorpion is considered the most dangerous scorpion in Brazil and is responsible for several cases of human envenomation annually. In this study, we performed transcriptome profiling of the T. serrulatus venom gland. In addition to transcripts with housekeeping functions, such as those related to protein synthesis, energy supply and structural processes, transcripts from thirty-five families of venom peptides or proteins were identified. These transcripts included three new complete sequences of toxins and more than a dozen putative venom gland proteins/peptides. The venom gland transcriptome profile was verified by comparison with the previously determined proteomic profile. In conclusion, this transcriptome data provides novel insights into the putative mechanisms underlying the venomous character of T. serrulatus. The collected data of scorpion transcripts and proteins/peptides described herein may be an important resource for identifying candidate targets of molecular therapies and preventative measures.The Tityus serrulatus scorpion is considered the most dangerous scorpion in Brazil and is responsible for several cases of human envenomation annually. In this study, we performed transcriptome profiling of the T. serrulatus venom gland. In addition to transcripts with housekeeping functions, such as those related to protein synthesis, energy supply and structural processes, transcripts from thirty-five families of venom peptides or proteins were identified. These transcripts included three new complete sequences of toxins and more than a dozen putative venom gland proteins/peptides. The venom gland transcriptome profile was verified by comparison with the previously determined proteomic profile. In conclusion, this transcriptome data provides novel insights into the putative mechanisms underlying the venomous character of T. serrulatus. The collected data of scorpion transcripts and proteins/peptides described herein may be an important resource for identifying candidate targets of molecular therapies and preventative measures.

关 键 词：SCORPIONS Antimicrobial Peptides Neutoxins VENOM GLANDS Brazilian Yellow SCORPION 

分 类 号：R73[医药卫生—肿瘤]