Structural Investigation of Cell Wall Mannan Antigen Obtained from Pathogenic Yeast <i>Candida zeylanoides</i>  

作　　者：Hidemitsu Kobayashi Susumu Kawakami Yukiko Ogawa Nobuyuki Shibata Shigeo Suzuki 

机构地区：[1]Department of Infection and Host Defense, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan [2]Laboratory of Microbiology, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Nagasaki, Japan [3]Sendai Research Institute for Mycology, Sendai, Miyagi, Japan

出　　处：《Open Journal of Medical Microbiology》2013年第2期139-143,共5页医学微生物学（英文）

摘　　要：Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor antibodies 1, 4, and 9, and react relatively weakly with factor antibody 5. We have investigated the structural characteristic of cell wall mannan of this strain using a non-degradable method, nuclear magnetic resonance analysis. The results obtained from NMR analysis, the two-dimensional homonuclear Hartmann-Hahn, indicated that C. zeylanoides mannan contains α-1, 3-linked and β-1,2-linked oligomannosyl side chains corresponding to C. guilliermondii specific antigenic factor 9. In addition, it was found that the mannan contains β-1,2-linked oligomannosyl side chains attached to phosphate groups corresponding to anitigenic factor 5, which were not found in C. guilliermondii mannan.Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor antibodies 1, 4, and 9, and react relatively weakly with factor antibody 5. We have investigated the structural characteristic of cell wall mannan of this strain using a non-degradable method, nuclear magnetic resonance analysis. The results obtained from NMR analysis, the two-dimensional homonuclear Hartmann-Hahn, indicated that C. zeylanoides mannan contains α-1, 3-linked and β-1,2-linked oligomannosyl side chains corresponding to C. guilliermondii specific antigenic factor 9. In addition, it was found that the mannan contains β-1,2-linked oligomannosyl side chains attached to phosphate groups corresponding to anitigenic factor 5, which were not found in C. guilliermondii mannan.

关 键 词：CANDIDA zeylanoides CANDIDA guilliermondii Cell Wall MANNAN Antigenic Factor Nuclear Magnetic Resonance 

分 类 号：R73[医药卫生—肿瘤]