A Comparative Study on the Biofilm Formation of Enterobacter agglomerans and Serretia rubideae in Different Environmental Parameter under Single Culture Condition  

作　　者：Jabun Naher Shahidul A. Chowdhury Abdullah A. Mamun Nuruddin Mahmud Wahhida Shumi Ruhul A. Khan 

机构地区：[1]Department of Microbiology, University of Chittagong, Chittagong, Bangladesh [2]Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh

出　　处：《Open Journal of Medical Microbiology》2014年第1期70-76,共7页医学微生物学（英文）

摘　　要：The biofilm formation by two opportunistic pathogens Enterobacter agglomerans and Serretia rubideae on different environmental parameter under single culture condition was compared. Biofilm clone formation unit (CFU) counting revealed that the number of Enterobacter was larger than Serretia after 24-h incubation. Consistent with this result, measurement of crystal violet staining of bacteria attached on the test tubes showed that the absorbance at 600 nm enhanced 2 times in Enterobacter than Serretia under optimum growth condition. Experiments with different environmental conditions such as incubation period, temperature, pH, NaCl concentration (aw) and medium ingredient contents for both organisms were carried out in Luria Bertani (LB) broth. The results indicated that Enterobacter grew well at 37?C after 24 h but Serretia grew well at 27?C after 48-h and 0.5% NaCl concentration was found optimum for both. On the other hand, 0.5% medium content and pH 5 was found optimum for Enterobacter whereas 2% medium content and pH 7 for Serretia.The biofilm formation by two opportunistic pathogens Enterobacter agglomerans and Serretia rubideae on different environmental parameter under single culture condition was compared. Biofilm clone formation unit (CFU) counting revealed that the number of Enterobacter was larger than Serretia after 24-h incubation. Consistent with this result, measurement of crystal violet staining of bacteria attached on the test tubes showed that the absorbance at 600 nm enhanced 2 times in Enterobacter than Serretia under optimum growth condition. Experiments with different environmental conditions such as incubation period, temperature, pH, NaCl concentration (aw) and medium ingredient contents for both organisms were carried out in Luria Bertani (LB) broth. The results indicated that Enterobacter grew well at 37?C after 24 h but Serretia grew well at 27?C after 48-h and 0.5% NaCl concentration was found optimum for both. On the other hand, 0.5% medium content and pH 5 was found optimum for Enterobacter whereas 2% medium content and pH 7 for Serretia.

关 键 词：PATHOGENS ENTEROBACTER agglomearns Serretia rubidaea BIOFILM NOSOCOMIAL INFECTION 

分 类 号：R73[医药卫生—肿瘤]