Genotypic Diversity and Characterization of Quinolone Resistant Determinants from Enterobacteriaceae in Yaoundé, Cameroon  

作　　者：Emilia Enjema Lyonga Mbamyah Michel Toukam Marie-Claire Okomo Assoumou Anthony M. Smith Celine Nkenfou Hortense Kamga Gonsu Anicette Chafa Betbeui Martha Tongo Mesembe Agnes Bedie Eyoh George Mondinde Ikomey Sinata Koulla-Shiro Emilia Enjema Lyonga Mbamyah;Michel Toukam;Marie-Claire Okomo Assoumou;Anthony M. Smith;Celine Nkenfou;Hortense Kamga Gonsu;Anicette Chafa Betbeui;Martha Tongo Mesembe;Agnes Bedie Eyoh;George Mondinde Ikomey;Sinata Koulla-Shiro(Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon;Centre for the Study and Control of Communicable Diseases, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon;Center for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa;Higher Teachers’ Training College, University of Yaoundé 1, Yaoundé, Cameroon;University Teaching Hospital, Yaoundé, Cameroon)

机构地区：[1]Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon [2]Centre for the Study and Control of Communicable Diseases, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon [3]Center for Enteric Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa [4]Higher Teachers’ Training College, University of Yaoundé 1, Yaoundé, Cameroon [5]University Teaching Hospital, Yaoundé, Cameroon

出　　处：《Open Journal of Medical Microbiology》2020年第2期33-45,共13页医学微生物学（英文）

摘　　要：<strong>Background:</strong> Enterobacteriaceae causes many types of infections which are often treated with quinolones and fluoroquinolone (Q/FQ). The resistance mechanisms to Q/FQ are usually associated with mutations in the quinolone resistance determining region which alter the conformation of target amino acid residues within the protein and in the <em>qnr</em> genes. This study aimed at determining the antimicrobial resistant profile of a sample of Enterobacteriaceae from Cameroon and the genetic diversity in quinolone-resistant isolates in view of implementing a better management, treatment, control and prevention of the transmission of these resistant strains. <strong>Methods:</strong> Identification and antimicrobial susceptibility testing was done using VITEK 2. The detection of plamid-mediated quinolone resistance (PMQR) genes was carried out using the conventional PCR method. Sequencing was done using the Applied Biosystem 3500 genetic analyser. DNA fingerprint was obtained using Pulsed-Field Gel electrophoresis. <strong>Results:</strong> Among 440 Enterobacteriaceae, the most prevalent genera were: <em>Escherichia</em> 178/440 (39.5%);<em>Klebsiella</em> 148/440 (33.6%);<em>Enterobacter </em>35/440 (8%);<em>Pantoea</em> 28/440 (6.4%);<em>Proteus</em> 14/440 (3.2%) <em>Salmonella </em>13/440 (3%). Ampicillin resistance showed the highest prevalence with 371/440 (81%) and Imipenem the lowest resistance 9/440 (2.1%). The ciprofloxacin resistance rate was 161/440 (36.6%). The detected plasmid mediated quinolone resistance (PMQR) genes were: <em>qnrA</em>, 2/161 (1.2%);<em>qnrB</em>, 31/161 (19.3%);<em>qnrS</em>, 13/161 (8.1%): <em>Aac</em> (6')<em>Ib-cr</em>, 84/161 (52.2%) and <em>qepA</em>, 3/161 (1.9%). There were several mutations in the <em>parC</em> gene of <em>Klebsiella</em> leading to S80D and S80N substitutions. Two pairs of <em>Klebsiella</em> <em>peumoniae</em> strains were phenotypically and genotypically identical with 100% similarity in the dendrogramme. <strong>Conclusion:</strong> This s<strong>Background:</strong> Enterobacteriaceae causes many types of infections which are often treated with quinolones and fluoroquinolone (Q/FQ). The resistance mechanisms to Q/FQ are usually associated with mutations in the quinolone resistance determining region which alter the conformation of target amino acid residues within the protein and in the <em>qnr</em> genes. This study aimed at determining the antimicrobial resistant profile of a sample of Enterobacteriaceae from Cameroon and the genetic diversity in quinolone-resistant isolates in view of implementing a better management, treatment, control and prevention of the transmission of these resistant strains. <strong>Methods:</strong> Identification and antimicrobial susceptibility testing was done using VITEK 2. The detection of plamid-mediated quinolone resistance (PMQR) genes was carried out using the conventional PCR method. Sequencing was done using the Applied Biosystem 3500 genetic analyser. DNA fingerprint was obtained using Pulsed-Field Gel electrophoresis. <strong>Results:</strong> Among 440 Enterobacteriaceae, the most prevalent genera were: <em>Escherichia</em> 178/440 (39.5%);<em>Klebsiella</em> 148/440 (33.6%);<em>Enterobacter </em>35/440 (8%);<em>Pantoea</em> 28/440 (6.4%);<em>Proteus</em> 14/440 (3.2%) <em>Salmonella </em>13/440 (3%). Ampicillin resistance showed the highest prevalence with 371/440 (81%) and Imipenem the lowest resistance 9/440 (2.1%). The ciprofloxacin resistance rate was 161/440 (36.6%). The detected plasmid mediated quinolone resistance (PMQR) genes were: <em>qnrA</em>, 2/161 (1.2%);<em>qnrB</em>, 31/161 (19.3%);<em>qnrS</em>, 13/161 (8.1%): <em>Aac</em> (6')<em>Ib-cr</em>, 84/161 (52.2%) and <em>qepA</em>, 3/161 (1.9%). There were several mutations in the <em>parC</em> gene of <em>Klebsiella</em> leading to S80D and S80N substitutions. Two pairs of <em>Klebsiella</em> <em>peumoniae</em> strains were phenotypically and genotypically identical with 100% similarity in the dendrogramme. <strong>Conclusion:</strong> This s

关 键 词：ENTEROBACTERIACEAE Quinolone Resistance Plasmid-Mediated Quinolone Resistance qnr Genes 

分 类 号：R73[医药卫生—肿瘤]