<i>In Vitro</i>Effects of Photodynamic Therapy with Three Photosensitizers on <i>Candida</i>spp from Complicated Vulvovaginitis and Asymptomatic Women  

作　　者：Humberto Fabio Boatto Maria Sayonara de Moraes Alexandre Paulo Machado Manoel Joã o Batista Castelo Girã o Gilglécia Novaes Pereira Santana Fernanda Aparecida Vieira Fernandes Orion Sant’anna Motter Borba Olga Fischman Gompertz Humberto Fabio Boatto;Maria Sayonara de Moraes;Alexandre Paulo Machado;Manoel João Batista Castelo Girão;Gilglécia Novaes Pereira Santana;Fernanda Aparecida Vieira Fernandes;Orion Sant’anna Motter Borba;Olga Fischman Gompertz(Faculty of Medicine, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil;Dermatologist of Sao Paulo State Secretary of Health, Sao Paulo, Brazil;Department of Basic Health Sciences, Faculty of Medicine, Federal University of Mato Grosso, Cuiabá, Brazil;Departament of Ginecology, Faculty of Medicine, Federal University of São Paulo, São Paulo, Brazil;Departament of Microbiology, Imunology e Parasitology, Federal University of São Paulo, São Paulo, Brazil)

机构地区：[1]Faculty of Medicine, University of Mogi das Cruzes, Mogi das Cruzes, Sã o Paulo, Brazil [2]Dermatologist of Sao Paulo State Secretary of Health, Sao Paulo, Brazil [3]Department of Basic Health Sciences, Faculty of Medicine, Federal University of Mato Grosso, Cuiabá, Brazil [4]Departament of Ginecology, Faculty of Medicine, Federal University of Sã o Paulo, Sã o Paulo, Brazil [5]Departament of Microbiology, Imunology e Parasitology, Federal University of Sã o Paulo, Sã o Paulo, Brazil

出　　处：《Open Journal of Medical Microbiology》2021年第4期283-297,共15页医学微生物学（英文）

摘　　要：<b><span style="font-family:Verdana;">Aim</span></b><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> To evaluate photodynamic therapy (PDT) </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> to reduce the growth of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp, and its synergy with the antifungals fluconazole and ketoconazole for inhibition of resistant, DDS and susceptible isolates from asymptomatic carriers and with complicated vulvovaginitis. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> Between 2017 and 2020</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> we evaluated 230 women with vulvovaginal candidiasis and 400 asymptomatic. We obtained 130 positive cultures for </span><i><span style="font-family:Verdana;">Candida </span></i><span style="font-family:Verdana;">spp from vulvovaginitis and 94 asymptomatic. Yeasts were characterized by classical and molecular tests. Sensitivity to fluconazole and ketoconazole was evaluated by E-test. We used photodynamic light through blue LED, wavelengths between 450 to 470 nm, power of 260 mW, energy</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">f</span><span style="font-family:Verdana;">l</span><span style="font-family:;" "=""><span style="font-family:Verdana;">uence of 270 J/cm</span>^{<span style="font-family:Verdana;">2</span>}<span style="font-family:Verdana;">, for 15 minutes over all colonies of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp</span></span><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">Methylene blue (MB) at 450 mg/mL, 2% gentian violet (VG) and 50</span><span style="font-family:;" "=""> </span><span style="font-fa<b><span style="font-family:Verdana;">Aim</span></b><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> To evaluate photodynamic therapy (PDT) </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> to reduce the growth of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp, and its synergy with the antifungals fluconazole and ketoconazole for inhibition of resistant, DDS and susceptible isolates from asymptomatic carriers and with complicated vulvovaginitis. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> Between 2017 and 2020</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> we evaluated 230 women with vulvovaginal candidiasis and 400 asymptomatic. We obtained 130 positive cultures for </span><i><span style="font-family:Verdana;">Candida </span></i><span style="font-family:Verdana;">spp from vulvovaginitis and 94 asymptomatic. Yeasts were characterized by classical and molecular tests. Sensitivity to fluconazole and ketoconazole was evaluated by E-test. We used photodynamic light through blue LED, wavelengths between 450 to 470 nm, power of 260 mW, energy</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">f</span><span style="font-family:Verdana;">l</span><span style="font-family:;" "=""><span style="font-family:Verdana;">uence of 270 J/cm</span>^{<span style="font-family:Verdana;">2</span>}<span style="font-family:Verdana;">, for 15 minutes over all colonies of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp</span></span><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">Methylene blue (MB) at 450 mg/mL, 2% gentian violet (VG) and 50</span><span style="font-family:;" "=""> </span><span style="font-fa

关 键 词：Photodynamic Therapy PHOTOSENSITIZERS Candida albicans Species Non-Albicans Complicated Fungal Vulvovaginitis 

分 类 号：O17[理学—数学]