Screening of Rodents from Rural Communities of Lagos and Ogun State Nigeria of Lassa Virus RNA  

作　　者：Fatai Sarafa Emmanuel Tochukwu Onyeje Joseph Ojonugwa Shaibu Olumuyiwa Babalola Salu Rosemary Ajuma Audu Sunday Aremu Omilabu Fatai Sarafa;Emmanuel Tochukwu Onyeje;Joseph Ojonugwa Shaibu;Olumuyiwa Babalola Salu;Rosemary Ajuma Audu;Sunday Aremu Omilabu(Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria;Centre for Human Virology and Genomics Research, Microbiology Department, Nigerian Institute of Medical Research, Lagos, Nigeria;Centre for Human and Zoonotic Virology, Central Research Laboratory and Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria)

机构地区：[1]Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria [2]Centre for Human Virology and Genomics Research, Microbiology Department, Nigerian Institute of Medical Research, Lagos, Nigeria [3]Centre for Human and Zoonotic Virology, Central Research Laboratory and Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria

出　　处：《Open Journal of Medical Microbiology》2024年第2期120-130,共11页医学微生物学（英文）

摘　　要：Background: In recent times, there has been an increase in the number of Lassa fever cases resulting from the several episodes of Lassa fever epidemics ravaging Nigeria and other West African countries. The presence of Lassa virus in rodents other than the major reservoir (Mastomys natalensis) has been a public health concern as to the actual burden of the disease. It is therefore of a public health necessity to explore the LASV RNA habouring potential of several species of rodents in endemic as well as non-endemic areas for proper prevention of emergence of outbreaks in non-endemic areas. Objectives: The aim of this study was to detect the presence of LASV RNA in different species of rodents in Ikorodu, Lagos state and Abeokuta, Ogun state. Methods: A total of ninety one (91) rodents were captured from Ikorodu, Lagos State (61 rodents) and Abeokuta, Ogun State (30 rodents), euthanized, bled, and plasma obtained for the detection of LASV RNA by Reverse Transcriptase Polymerase Chain Reaction. Results: A total of 91 rodents consisting of 77 Rattus rattus and 14 Crocidura spp. The S segment of LASV RNA was not in any of the 91 rodents’ plasma samples. Conclusion: The rodents captured within the rural communities of Ikorodu, Lagos State and Abeokuta, Ogun State were found not to habour the LASV RNA. This study is limited by the relatively small sample size. Similar studies should be encouraged both in endemic and non-endemic areas in order to understand the actual burden of Lassa fever as well as put into check future epidemics.Background: In recent times, there has been an increase in the number of Lassa fever cases resulting from the several episodes of Lassa fever epidemics ravaging Nigeria and other West African countries. The presence of Lassa virus in rodents other than the major reservoir (Mastomys natalensis) has been a public health concern as to the actual burden of the disease. It is therefore of a public health necessity to explore the LASV RNA habouring potential of several species of rodents in endemic as well as non-endemic areas for proper prevention of emergence of outbreaks in non-endemic areas. Objectives: The aim of this study was to detect the presence of LASV RNA in different species of rodents in Ikorodu, Lagos state and Abeokuta, Ogun state. Methods: A total of ninety one (91) rodents were captured from Ikorodu, Lagos State (61 rodents) and Abeokuta, Ogun State (30 rodents), euthanized, bled, and plasma obtained for the detection of LASV RNA by Reverse Transcriptase Polymerase Chain Reaction. Results: A total of 91 rodents consisting of 77 Rattus rattus and 14 Crocidura spp. The S segment of LASV RNA was not in any of the 91 rodents’ plasma samples. Conclusion: The rodents captured within the rural communities of Ikorodu, Lagos State and Abeokuta, Ogun State were found not to habour the LASV RNA. This study is limited by the relatively small sample size. Similar studies should be encouraged both in endemic and non-endemic areas in order to understand the actual burden of Lassa fever as well as put into check future epidemics.

关 键 词：Lassa Virus Lassa Fever Mastomys natalensis TRANSMISSION Lagos State Ogun State 

分 类 号：R73[医药卫生—肿瘤]