Identification of the autotransporter Pet toxin in Proteus mirabilis strain isolated from patients with urinary tract infections  

作　　者：Luis Raúl Gutiérrez-Lucas Guillermo Mendoza-Hernández Bertha González-Pedrajo Carlos Eslava-Campos Jaime Bustos-Martínez Teresita Sainz-Espunes 

机构地区：[1]Departamento de Sistemas Biológicos,Universidad Autónoma Metropolitana-Xochimilco,México City,México [2]Departamento de Bioquímica,Facultad de Medicina,UNAM,México City,México [3]Instituto de Fisiología Celular,UNAM,México City,México [4]Departamento de Atención a la Salud,Universidad Autónoma Metropolitana-Xochimilco,México City,México

出　　处：《Advances in Biological Chemistry》2012年第3期283-290,共8页生物化学进展（英文）

基　　金：supported by grant 171724 of CONACYT.

摘　　要：Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their own secretion across the outer membrane (type V autotransporter secretion mechanism). Serine protease autotransporters of Enterobacteriaceae (SPATEs) include adhesins, toxins, and proteases that can contribute to the virulence. Plasmid-encoded toxin (Pet) is the predominant protein in culture supernatants of enteroaggregative E. coli prototype strain 042 and has been extensively studied. Pet toxin is encoded on the 65-MDa adherence-related plasmid of EAEC 042 strain. In this work, Pet protein was found in the supernatant obtained from Proteus mirabilis RTX339 strain isolated from a psychiatric patient suffering complicated urinary tract infections (UTIs). The nucleotide sequence of pet gene was obtained using primers designed from E. coli 042 pet gene reported. The alignment of the sequence showed 100% identity with the pet gene reported. Is important to note that Proteus mirabilis RTX339 pet gene has chromosomal location. The chromosomal location of the gene was established since no plasmids were harbored by this strain.Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their own secretion across the outer membrane (type V autotransporter secretion mechanism). Serine protease autotransporters of Enterobacteriaceae (SPATEs) include adhesins, toxins, and proteases that can contribute to the virulence. Plasmid-encoded toxin (Pet) is the predominant protein in culture supernatants of enteroaggregative E. coli prototype strain 042 and has been extensively studied. Pet toxin is encoded on the 65-MDa adherence-related plasmid of EAEC 042 strain. In this work, Pet protein was found in the supernatant obtained from Proteus mirabilis RTX339 strain isolated from a psychiatric patient suffering complicated urinary tract infections (UTIs). The nucleotide sequence of pet gene was obtained using primers designed from E. coli 042 pet gene reported. The alignment of the sequence showed 100% identity with the pet gene reported. Is important to note that Proteus mirabilis RTX339 pet gene has chromosomal location. The chromosomal location of the gene was established since no plasmids were harbored by this strain.

关 键 词：PET Proteus mirabilis SPATEs 

分 类 号：R73[医药卫生—肿瘤]