Operational Protocol for Detection of Contamination by Actinides U,Pu and Am in Urine Using Calixarene Columns:From Mineralization to ICP-MS Measurement  

作　　者：Celine Bouvier-Capely Alexandre Legrand Aurelie Sylvain Annabelle Manoury Francois Rebiere 

机构地区：[1]Laboratoire de Radiochimie,Service de Dosimetrie Interne,PRP-HOM,Institut de Radioprotection et Surete Nucleaire(IRSN),Fontenay-aux-Roses,France

出　　处：《American Journal of Analytical Chemistry》2017年第5期317-333,共17页美国分析化学（英文）

摘　　要：Individual monitoring of workers exposed to the risk of intake of actinides requires suitable methods for measuring low level of excreted activity. The current protocols used for actinides analysis in bioassay are usually complicated and highly time consuming. In this work, a protocol based on the microwave digestion of urines followed by the separation of actinides using calix[6]arene-based chromatography columns and their measurement by a quadrupole ICP-MS is developed and validated, for the first time, on urine samples containing the three actinides, U, Pu and Am. With this protocol, the total analysis time is about 2 days, including the mineralization of urine and the chromatographic separation of actinides. Detection limits of actinides in urine are determined and compared to those obtained after “dilute and shoot” ICP-MS analysis or after alpha spectrometry measurement.Individual monitoring of workers exposed to the risk of intake of actinides requires suitable methods for measuring low level of excreted activity. The current protocols used for actinides analysis in bioassay are usually complicated and highly time consuming. In this work, a protocol based on the microwave digestion of urines followed by the separation of actinides using calix[6]arene-based chromatography columns and their measurement by a quadrupole ICP-MS is developed and validated, for the first time, on urine samples containing the three actinides, U, Pu and Am. With this protocol, the total analysis time is about 2 days, including the mineralization of urine and the chromatographic separation of actinides. Detection limits of actinides in urine are determined and compared to those obtained after “dilute and shoot” ICP-MS analysis or after alpha spectrometry measurement.

关 键 词：ACTINIDES URINE CALIX[6]ARENE Analysis ICP-MS 

分 类 号：R73[医药卫生—肿瘤]