Analysis of Bactericidal Components in Japanese Honeys  

作　　者：Koji Karasawa Masaru Kato Hidetoshi Arakawa Koji Karasawa;Masaru Kato;Hidetoshi Arakawa(Department of Bioanalytical Chemistry, School of Pharmacy, Showa University, Tokyo, Japan)

机构地区：[1]Department of Bioanalytical Chemistry, School of Pharmacy, Showa University, Tokyo, Japan

出　　处：《American Journal of Analytical Chemistry》2020年第8期309-321,共13页美国分析化学（英文）

摘　　要：It is known that the factors identified as contributing to the bactericidal activity of honeys are the high sugar concentration, hydrogen peroxide (H₂O₂) and low pH, and its bactericidal components depend on honey plant and natural environment. Manuka honey has been studied a lot about bactericidal effect. However, since Japanese honeys are mainly used as food, detailed analyzes of bactericidal components and its actions have not been reported. Therefore, we analyzed bactericidal components contained in nine Japanese honeys using Lucigenin-CL-HPLC. As our results, four species components of H₂O₂, glucose, fructose and methylglyoxal were detected from nine Japanese honeys. The H₂O₂ concentrations were 4.1 × 10^-5 - 1.8 × 10^-4 mol/L, the glucose concentrations were 1.4 - 2.8 mol/L, the fructose concentrations were 1.9 - 2.4 mol/L, the methylglyoxal concentrations were 4.0 × 10^-3 - 1.6 × 10^-2 mol/L. In this study, we confirmed that methylglyoxal is also contained in Japanese honeys, although it is a bactericidal component which is reported to be characteristic of manuka honey. It is considered that the further study of Japanese honeys is useful for more safe use, quality control, and clinical application.It is known that the factors identified as contributing to the bactericidal activity of honeys are the high sugar concentration, hydrogen peroxide (H₂O₂) and low pH, and its bactericidal components depend on honey plant and natural environment. Manuka honey has been studied a lot about bactericidal effect. However, since Japanese honeys are mainly used as food, detailed analyzes of bactericidal components and its actions have not been reported. Therefore, we analyzed bactericidal components contained in nine Japanese honeys using Lucigenin-CL-HPLC. As our results, four species components of H₂O₂, glucose, fructose and methylglyoxal were detected from nine Japanese honeys. The H₂O₂ concentrations were 4.1 × 10^-5 - 1.8 × 10^-4 mol/L, the glucose concentrations were 1.4 - 2.8 mol/L, the fructose concentrations were 1.9 - 2.4 mol/L, the methylglyoxal concentrations were 4.0 × 10^-3 - 1.6 × 10^-2 mol/L. In this study, we confirmed that methylglyoxal is also contained in Japanese honeys, although it is a bactericidal component which is reported to be characteristic of manuka honey. It is considered that the further study of Japanese honeys is useful for more safe use, quality control, and clinical application.

关 键 词：Japanese Honey Bactericidal Components Hydrogen Peroxide METHYLGLYOXAL Lucigenin-CL-HPLC 

分 类 号：R57[医药卫生—消化系统]