Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Exodeoxyribonuclease III from Crenarchaeon <i>Sulfolobus tokodaii</i>Strain 7  

作　　者：Shuichi Miyamoto Chieko Naoe Masaru Tsunoda Kazuo T. Nakamura 

机构地区：[1]Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto, Japan [2]Faculty of Pharmacy, Iwaki Meisei University, Iwaki, Japan [3]School of Pharmacy, Showa University, Tokyo, Japan

出　　处：《Crystal Structure Theory and Applications》2013年第4期155-158,共4页晶体结构理论与应用（英文）

摘　　要：Exodeoxyribonuclease III (EXOIII) acts as a 3’→5’ exonuclease and is homologous to purinic/apyrimidinic (AP) endonuclease (APE), which plays an important role in the base excision repair pathway. To structurally investigate the reaction and substrate recognition mechanisms of EXOIII, a crystallographic study of EXOIII from Sulfolobus tokodaii strain 7 was carried out. The purified enzyme was crystallized by using the hanging-drop vapor-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 154.2, b = 47.7, c = 92.4 ?, β = 125.8° and diffracted to 1.5 ? resolution.Exodeoxyribonuclease III (EXOIII) acts as a 3’→5’ exonuclease and is homologous to purinic/apyrimidinic (AP) endonuclease (APE), which plays an important role in the base excision repair pathway. To structurally investigate the reaction and substrate recognition mechanisms of EXOIII, a crystallographic study of EXOIII from Sulfolobus tokodaii strain 7 was carried out. The purified enzyme was crystallized by using the hanging-drop vapor-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 154.2, b = 47.7, c = 92.4 ?, β = 125.8° and diffracted to 1.5 ? resolution.

关 键 词：Crenarchaeon CRYSTALLIZATION Exodeoxyribonuclease SULFOLOBUS TOKODAII X-Ray Diffraction 

分 类 号：O6[理学—化学]