Validation of a Method for the Measurement of Caffeine in Water by HPLC-UV  

作　　者：Adel B. Shehata Abdulrahman R. AlAskar Mohammed A. AlRasheed Abdulrahman M. AlZahrany Fahd A. AlKharraa Sowailem A. AlSowailem Adel B. Shehata;Abdulrahman R. AlAskar;Mohammed A. AlRasheed;Abdulrahman M. AlZahrany;Fahd A. AlKharraa;Sowailem A. AlSowailem(Chemistry Department, National Measurement and Calibration Center (NMCC), Saudi Standards, Metrology and Quality Organization (SASO), Riyadh, Kingdom of Saudi Arabia)

机构地区：[1]Chemistry Department, National Measurement and Calibration Center (NMCC), Saudi Standards, Metrology and Quality Organization (SASO), Riyadh, Kingdom of Saudi Arabia

出　　处：《Green and Sustainable Chemistry》2023年第4期291-302,共12页绿色与可持续化学（英文）

摘　　要：For the production of a reference material from caffeine solution, one of the methods of characterization was HPLC-UV since caffeine is very sensitive to the UV. In this work, a batch solution of caffeine in water reference material of 1000 mg/kg has been gravimetrically prepared using a calibrated analytical balance. A sample of this solution was diluted to 25 mg/kg for measurement by HPLC-UV in the range 10 - 50 mg/kg. The chromatographic separation was carried out by C-18 column and a mobile phase assembled of 75% water and 25% methanol (v:v). The detection was made by the UV detector at 275 nm. The validation of this analytical method was carried out in accordance with requirements of the EURACHEM and ICH guidelines. The selectivity, linearity, accuracy, precision and trueness (recovery and bias) of the method were studied. The validation results proved that the method is fit-for-purpose of measuring the caffeine concentration in water in the range 10 - 50 mg/kg using HPLC-UV.For the production of a reference material from caffeine solution, one of the methods of characterization was HPLC-UV since caffeine is very sensitive to the UV. In this work, a batch solution of caffeine in water reference material of 1000 mg/kg has been gravimetrically prepared using a calibrated analytical balance. A sample of this solution was diluted to 25 mg/kg for measurement by HPLC-UV in the range 10 - 50 mg/kg. The chromatographic separation was carried out by C-18 column and a mobile phase assembled of 75% water and 25% methanol (v:v). The detection was made by the UV detector at 275 nm. The validation of this analytical method was carried out in accordance with requirements of the EURACHEM and ICH guidelines. The selectivity, linearity, accuracy, precision and trueness (recovery and bias) of the method were studied. The validation results proved that the method is fit-for-purpose of measuring the caffeine concentration in water in the range 10 - 50 mg/kg using HPLC-UV.

关 键 词：HPLC-UV CAFFEINE SELECTIVITY LINEARITY PRECISION ACCURACY 

分 类 号：R28[医药卫生—中药学]