Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry  

作　　者：Olga S. Brushinina Roman V. Gurto Maksim S. Timofeev Galina A. Frelikh Vladimir A. Slepichev Elena A. Yanovskaya Natalia U. Polomeeva Yulya G. Zyuz’kova Vladimir V. Udut 

机构地区：[1]Federal Governmental Budgetary Institution “Research Institute of Pharmacology E.D. Goldberg’s Name” Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia [2]Federal Governmental Budgetary Institution “Research Institute of Pharmacology E.D. Goldberg’s Name”Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia

出　　处：《International Journal of Analytical Mass Spectrometry and Chromatography》2014年第2期25-32,共8页分析质谱与色谱期刊（英文）

摘　　要：Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—negative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—negative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.

关 键 词：CANDESARTAN HYDROCHLOROTHIAZIDE LC-MS PHARMACOKINETIC 

分 类 号：O6[理学—化学]