3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents  

作　　者：Dmitry E. Pugachev Georgy V. Zatonsky Tatyana S. Kostryukova Anna G. Shubina Nikolay V. Vasiliev Dmitry E. Pugachev;Georgy V. Zatonsky;Tatyana S. Kostryukova;Anna G. Shubina;Nikolay V. Vasiliev(Department of Biological Microanalysis, FGUP State Research Institute of Biological Engineering, Moscow, Russia;Department of Theoretical and Applied Chemistry, State University of Education, Mytishchi, Russia)

机构地区：[1]Department of Biological Microanalysis, FGUP State Research Institute of Biological Engineering, Moscow, Russia [2]Department of Theoretical and Applied Chemistry, State University of Education, Mytishchi, Russia

出　　处：《International Journal of Organic Chemistry》2024年第1期20-31,共12页有机化学国际期刊（英文）

摘　　要：New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu³⁺ ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu³⁺ ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.

关 键 词：Fluorescence Immunoassay Fluorinated β-Diketones CARBAZOLE Europium Complexes STREPTAVIDIN Nanodispersions 

分 类 号：O62[理学—有机化学]