Limonene’s Bacteriostatic Activity against Ralstonia solanacearum by Compromised Membrane Integrity: A Transcriptomic and Metabolomic Analysis  

作　　者：Ruya Zhang Honghao Zhang Linfeng Li Yuchen Feng Lin Yang Jiaxin Liu Senfeng Gao Zihao Zhou Yong Yang Haibo Xiang Mei Li Jun Yu Ruya Zhang;Honghao Zhang;Linfeng Li;Yuchen Feng;Lin Yang;Jiaxin Liu;Senfeng Gao;Zihao Zhou;Yong Yang;Haibo Xiang;Mei Li;Jun Yu(College of Life Sciences, Hubei University, Wuhan, China;State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Sciences, Hubei University, Wuhan, China;Tobacco Research Institute of Hubei Province, Wuhan, China)

机构地区：[1]College of Life Sciences, Hubei University, Wuhan, China [2]State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Sciences, Hubei University, Wuhan, China [3]Tobacco Research Institute of Hubei Province, Wuhan, China

出　　处：《Journal of Agricultural Chemistry and Environment》2024年第3期312-324,共13页农业化学和环境（英文）

摘　　要：Ralstonia solanacearum, the causative agent of bacterial wilt, is a soil-borne pathogen that poses a widespread threat to plants in the Solanaceae family. To elucidate the mechanism by which limonene exerts its effects on R. solanacearum, we first assessed the impact of limonene on the physiological indicators of the pathogen and subsequently analyzed its transcriptome and metabolome. Our findings indicate that limonene has a potent inhibitory effect on R. solanacearum, and it also suppresses the formation of the bacterial community biofilm. Limonene primarily regulates the terpene biosynthesis pathway in R. solanacearum, thereby potentially affecting signal transduction in the pathogen and disrupting its normal growth and development. These results significantly enhance our understanding of limonene’s response to the induction of bacterial wilt and provide a reference for further prevention and control of R. solanacearum.Ralstonia solanacearum, the causative agent of bacterial wilt, is a soil-borne pathogen that poses a widespread threat to plants in the Solanaceae family. To elucidate the mechanism by which limonene exerts its effects on R. solanacearum, we first assessed the impact of limonene on the physiological indicators of the pathogen and subsequently analyzed its transcriptome and metabolome. Our findings indicate that limonene has a potent inhibitory effect on R. solanacearum, and it also suppresses the formation of the bacterial community biofilm. Limonene primarily regulates the terpene biosynthesis pathway in R. solanacearum, thereby potentially affecting signal transduction in the pathogen and disrupting its normal growth and development. These results significantly enhance our understanding of limonene’s response to the induction of bacterial wilt and provide a reference for further prevention and control of R. solanacearum.

关 键 词：LIMONENE Transcriptome METABOLOME Ralstonia solanacearum Terpenoid Backbone Biosynthesis 

分 类 号：Q93[生物学—微生物学]