Rapid Separation of Five Cyclosporin Analogs by Supercritical Fluid Chromatography  

作　　者：Yuefei Shao Chunlei Wang Atsu Apedo Oliver Mcconnell Yuefei Shao;Chunlei Wang;Atsu Apedo;Oliver Mcconnell(Bioanalytical and Discovery Analytical Sciences, Research & Development, Bristol-Myers Squibb, Princeton, New Jersey, USA)

机构地区：[1]Bioanalytical and Discovery Analytical Sciences, Research & Development, Bristol-Myers Squibb, Princeton, New Jersey, USA

出　　处：《Journal of Analytical Sciences, Methods and Instrumentation》2016年第2期23-32,共10页分析科学方法和仪器期刊（英文）

摘　　要：Recently there has been a resurgence of interest in cyclic peptides due to their therapeutic advantages in terms of potency, permeability, proteolytic stability, and unique selectivity relative to traditional smaller drug molecules. Cyclosporin is a family of cyclic peptides widely used as autoimmune suppression agents. Cyclosporin analogs consist of eleven amino acids with the main difference lying at the side chain of its amino acid residues. In this study, a single step separation method was developed utilizing Supercritical Fluid Chromatography (SFC) to resolve five naturally occurring cyclosporin analogs (Cyclosporin A, B, C, D, and H) on a bare silica-packed column. The optimized method involved use of ethanol-modified carbon dioxide as mobile phase on a bare silica column at 80 °C and UV detection at 220 nm. Although column temperature and back pressure generally had insignificant effect on SFC separation, it was found in our study that increasing temperature and pressure greatly improved peak shape and resolution.Recently there has been a resurgence of interest in cyclic peptides due to their therapeutic advantages in terms of potency, permeability, proteolytic stability, and unique selectivity relative to traditional smaller drug molecules. Cyclosporin is a family of cyclic peptides widely used as autoimmune suppression agents. Cyclosporin analogs consist of eleven amino acids with the main difference lying at the side chain of its amino acid residues. In this study, a single step separation method was developed utilizing Supercritical Fluid Chromatography (SFC) to resolve five naturally occurring cyclosporin analogs (Cyclosporin A, B, C, D, and H) on a bare silica-packed column. The optimized method involved use of ethanol-modified carbon dioxide as mobile phase on a bare silica column at 80 °C and UV detection at 220 nm. Although column temperature and back pressure generally had insignificant effect on SFC separation, it was found in our study that increasing temperature and pressure greatly improved peak shape and resolution.

关 键 词：Cyclic Peptide CYCLOSPORIN Supercritical Fluid Chromatography 

分 类 号：O62[理学—有机化学]