Influence of Ozonized Oil Nanoemulsions on B-16 Melanoma Cells: An <i>in Vitro Study</i>  

作　　者：Yeliz Yalçın Ishak Özel Tekin R. Seda Tığlı Aydın Yeliz Yalçın;Ishak Özel Tekin;R. Seda Tığlı Aydın(Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey;Department of Immunology, Facultyof Medicine, ZonguldakBülent Ecevit University, Zonguldak, Turkey;Department of Biomedical Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey)

机构地区：[1]Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey [2]Department of Immunology, Facultyof Medicine, ZonguldakBülent Ecevit University, Zonguldak, Turkey [3]Department of Biomedical Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey

出　　处：《Journal of Biomaterials and Nanobiotechnology》2021年第4期49-56,共8页生物材料与纳米技术（英文）

摘　　要：In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B-16 melanoma cell line which were incubated with various OZNE doses (v/v). Antitumor effects of OZNE against cancer cell lines were evaluated by cellular morphology, apoptosis and cell cycle analysis. Flow cytometry results showed that OZNE induced DNA damage, apoptosis, and arrested cell cycle in G0-1 phase in B-16 melanoma cells. Thus, OZNE treatment could pose an effective way to act as a potential therapeutic for patients with tumors in the future.In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B-16 melanoma cell line which were incubated with various OZNE doses (v/v). Antitumor effects of OZNE against cancer cell lines were evaluated by cellular morphology, apoptosis and cell cycle analysis. Flow cytometry results showed that OZNE induced DNA damage, apoptosis, and arrested cell cycle in G0-1 phase in B-16 melanoma cells. Thus, OZNE treatment could pose an effective way to act as a potential therapeutic for patients with tumors in the future.

关 键 词：Ozonized Oil NANOEMULSION B-16 Melanoma Cancer 

分 类 号：R73[医药卫生—肿瘤]