In Vitro Evaluation of Two Tissue Substitutes for Gingival Augmentation  

作　　者：Tulio Cardoso dos Santos Carlos Nelson Elias Alexandre Barboza Lemos Andresa Borges Soares André Antonio Pelegrine Lucas Novaes Teixeira Elizabeth Ferreira Martinez Tulio Cardoso dos Santos;Carlos Nelson Elias;Alexandre Barboza Lemos;Andresa Borges Soares;André Antonio Pelegrine;Lucas Novaes Teixeira;Elizabeth Ferreira Martinez(Division of Oral Implantology, Faculdade Sã,o Leopoldo Mandic, Campinas, SP, Brazil;Department of Materials Science, Military Institute of Engineering (IME), Rio de Janeiro, RJ, Brazil;Division of Oral Pathology, Faculdade Sã,o Leopoldo Mandic, Campinas, SP, Brazil;Division of Cell Biology, Faculdade Sã,o Leopoldo Mandic, Campinas, SP, Brazil)

机构地区：[1]Division of Oral Implantology, Faculdade Sã,o Leopoldo Mandic, Campinas, SP, Brazil [2]Department of Materials Science, Military Institute of Engineering (IME), Rio de Janeiro, RJ, Brazil [3]Division of Oral Pathology, Faculdade Sã,o Leopoldo Mandic, Campinas, SP, Brazil [4]Division of Cell Biology, Faculdade Sã,o Leopoldo Mandic, Campinas, SP, Brazil

出　　处：《Journal of Biomaterials and Nanobiotechnology》2023年第2期23-34,共12页生物材料与纳米技术（英文）

摘　　要：Three-dimensional collagen matrices of porcine origin are being used as substitutes for soft tissue grafts in periodontal plastic surgery in search of aesthetic and natural results. This in vitro study aimed to compare Fibro-Gide® (GeistlichBiomaterials) and Mucoderm® (BotissBiomaterials) matrices during the initial phase of soft tissue formation. For this purpose, samples of 5 × 5 mm were obtained, and then human fibroblasts were plated on them. After 24, 48 and 72 h, cell viability was assessed using an MTT assay, and the secretion of type I collagen, MMP-2, TIMP-1 and TIMP-2 was analyzed by ELISA immunoassay. The control group (C) consisted of cells plated on polystyrene without the matrices. The morphology of the surfaces was also examined using scanning electron microscopy (SEM), as was the average roughness (Ra) of the samples by a profilometer. Topographic analysis revealed that roughness was significantly higher on Mucoderm® than on Fibro-Gide® (p 0.05). The synthesis of type I collagen, MMP-2 and TIMP-1 were significantly higher from cells plated on Fibro-Gide® than on Mucoderm®, in all time points (p ® than on Mucoderm® (p ® induced an increase in type I collagen, MMP-2 and TIMP-1 and TIMP-2.Three-dimensional collagen matrices of porcine origin are being used as substitutes for soft tissue grafts in periodontal plastic surgery in search of aesthetic and natural results. This in vitro study aimed to compare Fibro-Gide® (GeistlichBiomaterials) and Mucoderm® (BotissBiomaterials) matrices during the initial phase of soft tissue formation. For this purpose, samples of 5 × 5 mm were obtained, and then human fibroblasts were plated on them. After 24, 48 and 72 h, cell viability was assessed using an MTT assay, and the secretion of type I collagen, MMP-2, TIMP-1 and TIMP-2 was analyzed by ELISA immunoassay. The control group (C) consisted of cells plated on polystyrene without the matrices. The morphology of the surfaces was also examined using scanning electron microscopy (SEM), as was the average roughness (Ra) of the samples by a profilometer. Topographic analysis revealed that roughness was significantly higher on Mucoderm® than on Fibro-Gide® (p 0.05). The synthesis of type I collagen, MMP-2 and TIMP-1 were significantly higher from cells plated on Fibro-Gide® than on Mucoderm®, in all time points (p ® than on Mucoderm® (p ® induced an increase in type I collagen, MMP-2 and TIMP-1 and TIMP-2.

关 键 词：Collagen Matrices Gingival Recession Periodontal Regeneration Matrix Metalloproteinases 

分 类 号：R73[医药卫生—肿瘤]