The Biochemical Impact by Covalent Shielding of the Anionic Oxygen of the Phosphate Group in DNA and RNA as Methylated Phosphotriester Linkage on the Inhibition of DNA Duplication and on HIV-1 RNA Viral Infectivity Has Been Seriously Overlooked  

作　　者：Henk M. Buck Henk M. Buck(Kasteel Twikkelerf 94, Tilburg, The Netherlands)

机构地区：[1]Kasteel Twikkelerf 94, Tilburg, The Netherlands

出　　处：《Journal of Biophysical Chemistry》2023年第2期59-66,共8页生物物理化学（英文）

摘　　要：With the help of model experiments, we are able to offer a detailed proposal for the inhibition of DNA duplication and no inhibition of RNA viral infectivity. As a backbone, we introduced methyl phosphotriester (MPTE). Duplex formation according to the traditional Watson and Crick base-pairing: [(MPTE)_n−1 DNA] * DNA and [(MPTE)_n−1 DNA] * RNA, where n = number of DNA and RNA bases. However, in the latter case, inhibition is obtained by reduction of the number of MPTE linkages, as is confirmed with model experiments and under biological conditions with micro (mi)RNA substrates. The latter results have recently been published. One or more single MPTEs are disseminated over different places of DNA without neighbour MPTEs (Prof. Wen-Yih Chen and his group, Taiwan).With the help of model experiments, we are able to offer a detailed proposal for the inhibition of DNA duplication and no inhibition of RNA viral infectivity. As a backbone, we introduced methyl phosphotriester (MPTE). Duplex formation according to the traditional Watson and Crick base-pairing: [(MPTE)_n−1 DNA] * DNA and [(MPTE)_n−1 DNA] * RNA, where n = number of DNA and RNA bases. However, in the latter case, inhibition is obtained by reduction of the number of MPTE linkages, as is confirmed with model experiments and under biological conditions with micro (mi)RNA substrates. The latter results have recently been published. One or more single MPTEs are disseminated over different places of DNA without neighbour MPTEs (Prof. Wen-Yih Chen and his group, Taiwan).

关 键 词：Methylated Phosphotriester (MPTE) DNA Partially MPTE DNA Model Inhibition Experiments Micro (mi)RNA HIV-1 RNA Conformational Transition 

分 类 号：R73[医药卫生—肿瘤]