Electrochemical Sensor for Dopamine Detection Based on the CRISPR/Cas12 System  

作　　者：Liu Huang Hongqu Liang Binyu Bi Weijuan Yan Jun Zhou Xuebin Li Liu Huang;Hongqu Liang;Binyu Bi;Weijuan Yan;Jun Zhou;Xuebin Li(Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China;Clinical Medicine School of Youjiang Medical University for Nationalities, Baise, China)

机构地区：[1]Department of Neurology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China [2]Clinical Medicine School of Youjiang Medical University for Nationalities, Baise, China

出　　处：《Materials Sciences and Applications》2024年第11期528-537,共24页材料科学与应用期刊（英文）

摘　　要：Dopamine is an important neurotransmitter and biomarker that is involved in many physiological processes in the body as well as the control of the central nervous system. Therefore, it is crucial to accurately monitor dopamine concentrations in organisms in order to comprehend their biological roles and make correct clinical diagnoses. In this work, we describe the development of an aptamer sensor utilizing gold electrodes and cyclic voltammetry. Using a self-assembly approach, a single-chain sulfhydrylated dopamine-specific aptamer was immobilized on the surface of a gold electrode to successfully create the aptamer sensor. Voltammetry was used to do a thorough electrochemical characterization in order to assess the sensor’s performance. According to the findings, the created electrochemical sensor demonstrated outstanding analytical capabilities for the detection of dopamine, including a wide linear response range, a very low detection limit, high sensitivity, and great selectivity. These characteristics make the sensor a novel approach for the quick and precise detection of dopamine, and it is anticipated that clinical diagnostics and biological research will use it extensively.Dopamine is an important neurotransmitter and biomarker that is involved in many physiological processes in the body as well as the control of the central nervous system. Therefore, it is crucial to accurately monitor dopamine concentrations in organisms in order to comprehend their biological roles and make correct clinical diagnoses. In this work, we describe the development of an aptamer sensor utilizing gold electrodes and cyclic voltammetry. Using a self-assembly approach, a single-chain sulfhydrylated dopamine-specific aptamer was immobilized on the surface of a gold electrode to successfully create the aptamer sensor. Voltammetry was used to do a thorough electrochemical characterization in order to assess the sensor’s performance. According to the findings, the created electrochemical sensor demonstrated outstanding analytical capabilities for the detection of dopamine, including a wide linear response range, a very low detection limit, high sensitivity, and great selectivity. These characteristics make the sensor a novel approach for the quick and precise detection of dopamine, and it is anticipated that clinical diagnostics and biological research will use it extensively.

关 键 词：CRISPR/Cas12 DOPAMINE Electrochemical Biosensor 

分 类 号：TP2[自动化与计算机技术—检测技术与自动化装置]