Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening  

作　　者：Anna Segecova Jan Cerveny Thomas Roitsch 

机构地区：[1]Department of Adaptive Biotechnologies,Global Change Research Institute,CAS,Drasov,Czech Republic [2]Research Centre for Toxic Compounds in the Environment(RECETOX),Masaryk University,Brno,Czech Republic [3]Departmemt for Plant and Environmental Sciences,Copenhagen Plant Science Centre,University of Copenhagen,Taastrup,Denmark

出　　处：《Journal of Environmental Protection》2017年第6期678-692,共15页环境保护（英文）

基　　金：supported by the Ministry of Education,Youth and Sports of CR within the National Sustainability Program I(NPU I),grant number LO1415;supported by GA CR,grant number 15-17367S;supported by the Czech research infrastructure for systems biology C4SYS(project no LM2015055).

摘　　要：Photoautotrophic suspension cultures have been established from various model and crop plants and proved to be valuable and robust experimental system to assess coordinated responses of primary and secondary metabolism to metabolic and stress related signals. The use of suspension cultures combines the ease of handling microalgae in microtiter plates with the advantage of testing physiological responses of higher plants, notably in combination with the assessment of the response of photosynthetic activity by PAM chlorophyll fluorescence imaging as well as monitoring changes in secondary metabolite production and ROS formation by steady state fluorescence of plant fluorophores or introduced fluorescent probes. Photoautotrophic cultures provide various advantages as fast, highly sensitive, robust and high-through-put experimental system for screening and characterization of the impact of toxic compounds on higher plants. This opinion article discusses and critically evaluates the potential of photoautotrophic cultures of higher plants in combination with fluorescence imaging assays in microtiter plates as a complement to existing guidelines for testing the toxicity of chemicals in plants.Photoautotrophic suspension cultures have been established from various model and crop plants and proved to be valuable and robust experimental system to assess coordinated responses of primary and secondary metabolism to metabolic and stress related signals. The use of suspension cultures combines the ease of handling microalgae in microtiter plates with the advantage of testing physiological responses of higher plants, notably in combination with the assessment of the response of photosynthetic activity by PAM chlorophyll fluorescence imaging as well as monitoring changes in secondary metabolite production and ROS formation by steady state fluorescence of plant fluorophores or introduced fluorescent probes. Photoautotrophic cultures provide various advantages as fast, highly sensitive, robust and high-through-put experimental system for screening and characterization of the impact of toxic compounds on higher plants. This opinion article discusses and critically evaluates the potential of photoautotrophic cultures of higher plants in combination with fluorescence imaging assays in microtiter plates as a complement to existing guidelines for testing the toxicity of chemicals in plants.

关 键 词：TOXINS TOXICANTS ECOTOXICOLOGY PAM Chlorophyll Fluorescence 

分 类 号：R73[医药卫生—肿瘤]