Variability of Deltamethrin-Resistant <i>Metarhizium anisopliae</i>Aggressive Strains Group to a Population of the Cossid Moth from <i>Eucalyptus nitens</i>  

作　　者：Pedro Romón Hardus Hatting Arturo Goldarazena Juan Carlos Iturrondobeitia 

机构地区：[1]Department of Zoology and Animal Cell Biology, UPV-EHU, University of Basque Country, Leioa, Spain [2]FABI, Forestry and Agricultural Biotechnology Institute, TPCP, Tree Protection Co-Operative Programme, University of Pretoria, Pretoria, South Africa [3]ELIB, Earth and Life Institute, Université catholique de Louvain, Louvain, Belgium

出　　处：《Open Journal of Forestry》2018年第2期196-204,共9页林学期刊（英文）

摘　　要：Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.

关 键 词：Coryphodema tristis METARHIZIUM anisopliae Assays Compatibility with DELTAMETHRIN RAPDS 

分 类 号：R73[医药卫生—肿瘤]