Impact of Yupingfengsan Oral Liquid on the Expression of IL-4, IFN-<i>γ</i>and NF-<i>κ</i>B of Allergic Rhinitis Mice  

作　　者：Benyuan Chen Chenggang Mao Yating Wan Lijia Wan Benyuan Chen;Chenggang Mao;Yating Wan;Lijia Wan(The Second Clinical Medical College of Yangtze University, Jingzhou, China;Otolaryngology Head and Neck Surgery, Jingzhou Central Hospital, Jingzhou, China)

机构地区：[1]The Second Clinical Medical College of Yangtze University, Jingzhou, China [2]Otolaryngology Head and Neck Surgery, Jingzhou Central Hospital, Jingzhou, China

出　　处：《Yangtze Medicine》2021年第2期125-132,共8页长江医药（英文）

摘　　要：To observe the impact of Yupingfengsan Oral Liquid on the expression of IL-4 and IFN-<em>γ</em> in AR mice’s serum and expression level of nuclear factor—<em>κ</em>B (NF-<em>κ</em>B) protein and gene in nasal mucosa. <strong>Method:</strong> Forty BALB/c mice were randomly divided into the normal group, model group, Yupingfengsan Oral Liquid group (6 g/kg) and Loratadine group, with 10 mice per group. AR mice model was established by OVA, and IL-4 and IFN-<em>γ</em> contents can be measured with ELISA. The morphological changes of nasal mucosa were observed by hematoxylin eosin (HE) staining and NF-<em>κ</em>B expression in the nasal mucosa of mice was tested with Real-Time PCR and Western blot. <strong>Results:</strong> Compared with the model group, the nasal symptoms in the Yupingfengsan Oral Liquid group and Loratadine group were obviously relieved. HE staining showed that there was a little inflammatory cell infiltration in the nasal mucosa of Yupingfengsan Oral Liquid group and Loratadine group and it was significantly reduced when compared with the model group. IL-4 level in the serum and expression of NF-<em>κ</em>B protein and gene in the nasal mucosa was consistent and it was decreased when compared with the model group (P < 0.01), but the IFN-<em>γ</em> level in the serum was increased (P < 0.01). <strong>Conclusion:</strong> Yupingfengsan Oral Liquid can improve the clinical symptoms and histopathological manifestations of AR mice sensitized by OVA, inhibit the NF-<em>κ</em>B expression, balance the percentage of Th1/Th2 cells, increase the IFN-<em>γ</em> level in the serum and decrease the IL-4 level.To observe the impact of Yupingfengsan Oral Liquid on the expression of IL-4 and IFN-<em>γ</em> in AR mice’s serum and expression level of nuclear factor—<em>κ</em>B (NF-<em>κ</em>B) protein and gene in nasal mucosa. <strong>Method:</strong> Forty BALB/c mice were randomly divided into the normal group, model group, Yupingfengsan Oral Liquid group (6 g/kg) and Loratadine group, with 10 mice per group. AR mice model was established by OVA, and IL-4 and IFN-<em>γ</em> contents can be measured with ELISA. The morphological changes of nasal mucosa were observed by hematoxylin eosin (HE) staining and NF-<em>κ</em>B expression in the nasal mucosa of mice was tested with Real-Time PCR and Western blot. <strong>Results:</strong> Compared with the model group, the nasal symptoms in the Yupingfengsan Oral Liquid group and Loratadine group were obviously relieved. HE staining showed that there was a little inflammatory cell infiltration in the nasal mucosa of Yupingfengsan Oral Liquid group and Loratadine group and it was significantly reduced when compared with the model group. IL-4 level in the serum and expression of NF-<em>κ</em>B protein and gene in the nasal mucosa was consistent and it was decreased when compared with the model group (P < 0.01), but the IFN-<em>γ</em> level in the serum was increased (P < 0.01). <strong>Conclusion:</strong> Yupingfengsan Oral Liquid can improve the clinical symptoms and histopathological manifestations of AR mice sensitized by OVA, inhibit the NF-<em>κ</em>B expression, balance the percentage of Th1/Th2 cells, increase the IFN-<em>γ</em> level in the serum and decrease the IL-4 level.

关 键 词：Allergic Rhinitis (AR) Yupingfeng Powder Oral Solution Nuclear Factor-κB (NF-κB) Interleukin-4 (IL-4) γ-Interferon (INF-γ) 

分 类 号：R73[医药卫生—肿瘤]