国家自然科学基金(31272015)

作品数:5被引量:33H指数:4
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相关作者:吴建祥周雪平李娜任春梅刘欢更多>>
相关机构:浙江大学江苏省农业科学院更多>>
相关期刊:《中国农业科学》《Journal of Integrative Agriculture》《农业生物技术学报》《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》更多>>
相关主题:DOT-ELISAIC-RT-PCRMONOCLONAL_ANTIBODY单克隆抗体DAS-ELISA更多>>
相关领域:农业科学更多>>
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Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus被引量:7
《Journal of Integrative Agriculture》2017年第1期115-124,共10页CHEN Zhe ZHANG Ming-hao ZHOU Xue-ping WU Jian-xiang 
supported by the National Natural Science Foundation of China(31272015);the National Basic Research Program(973)of China(2014CB138400);the Special Fund for Agro-scientific Research in the Public Interest,China(201303021,201303028)
Aphid-borne Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection techniq...
关键词:Zucchini yellow mosaic virus monoclonal antibody ACP-ELISA DOT-ELISA tissue blot-ELISA DAS-ELISA IC-RT-PCR 
西瓜花叶病毒(WMV)单克隆抗体的制备及其应用被引量:4
《中国农业科学》2016年第14期2711-2724,共14页陈浙 宋革 周雪平 吴建祥 
国家自然科学基金(31272015);国家公益性行业(农业)科研专项(201203076-05)
【目的】制备抗西瓜花叶病毒(Watermelon mosaic virus,WMV)的特异性单克隆抗体,并以其为核心建立能快速有效地检测WMV的血清学方法,从而为中国田间西瓜花叶病毒病的诊断和检测、预测预警及科学防控体系的建立提供物质和技术支撑。【方...
关键词:西瓜花叶病毒(WMV) 单克隆抗体 ACP-ELISA DAS-ELISA DOT-ELISA Tissue blot-ELISA IC-RT-PCR 
中国小麦花叶病毒(CWMV)单克隆抗体制备及其检测应用被引量:13
《农业生物技术学报》2015年第6期711-719,共9页刘欢 李娜 周雪平 任春梅 程兆榜 吴建祥 
公益性行业(农业)科研专项项目(No.201303021);国家自然科学基金面上项目(No.31272015)
中国小麦花叶病是引起小麦(Triticum aestivum)严重减产的中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)造成的。本研究旨在以制备的抗CWMV特异性单克隆抗体(monoclonal antibody,MAb)为核心建立检测CWMV的血清学方法,为该病毒病...
关键词:中国小麦花叶病毒(CWMV) 单克隆抗体(MAb) 抗原包被酶联免疫吸附试验(ACP-ELISA) 斑点酶联免疫吸附试验(dot-ELISA) 
Genetic variability and evolution of rice stripe virus
《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第10期875-885,共11页Ling-zhe HUANG Li-xia RAO Xue-ping ZHOU Jian-xiang WU 
Project supported by the National Natural Science Foundation of China(No.31272015);the National Basic Research Program (973) of China(No.2010CB126203);the Earmarked Fund for Modern Agro-industry Technology Research System of Ministry of Agriculture of China;the Zhejiang Provincial Natural Science Foundation of China(No.Z3090039)
Rice stripe virus(RSV) is the type member of the genus Tenuivirus.RSV is known to have four segmented,single-stranded RNA molecules and causes rice stripe disease in the rice fields of China,Japan,and Korea.Based on t...
关键词:Rice stripe virus Genetic variability Genetic evolution 
Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China被引量:11
《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第7期555-562,共8页Jian-xiang WU Qiang WANG Huan LIU Ya-juan QIAN Yan XIE Xue-ping ZHOU 
supported by the National Natural Science Foundation of China (No. 31272015);the Ministry of Education of China (No. 313052);the Zhejiang Provincial Natural Science Foundation of China (No. Z3090039)
Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (...
关键词:Maize chlorotic mottle virus (MCMV) Immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) Triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) Monoclonal antibody (MAb) Dot-immunobinding assay (DIBA) 
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