国家自然科学基金(30471527)

作品数:3被引量:1H指数:1
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相关作者:钱冬萌王斌丁守怡闫志勇宋旭霞更多>>
相关机构:青岛大学更多>>
相关期刊:《Journal of Microbiology and Immunology》《Chinese Journal of Cancer Research》更多>>
相关主题:SELDICELL_LINEPROTEIN_EXPRESSION差异蛋白肝肿瘤更多>>
相关领域:生物学医药卫生更多>>
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Differential Proteomics in Malignant and Normal Liver Cell Lines
《Chinese Journal of Cancer Research》2007年第2期94-99,共6页刘志军 王斌 闫志勇 钱冬萌 宋旭霞 丁守怡 白志强 
the grant from the National Natural Science Foundation of China(No.30471527, No. 30540075);Mt. Tai Scholar Construction Engineering Foundation
Objective: To detect differential protein expression in malignant and normal liver cell lines in vitro using the SELDI ProteinChip platform, for investigating the pathogenesis of liver cancer. Methods: Two cell line...
关键词:SELDI ProteinChip Liver cancer cell line SMMC-7721 Normal liver cell line L02 Protein expression 
Proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus,as demonstrated by the surface enhanced laser desorption/ionization(SELDI)protein chip system
《Journal of Microbiology and Immunology》2007年第1期46-51,共6页ZHI JUN LIU BIN WANG YING TIAN ZHI QIANG BAI SHOU YI DING Xu XIA SONG ZHI YONG YAN DONG MENG QIAN 
This work was supported by National Natural Science Foundation of China(No.30471527 and No.30540075);partly supported by Mr.Tai Scholar Construction Engineering Foundation.
The proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus (HCMV) was investigated at the protein level by using the surface enhanced laser desorp...
关键词:SELDI protein chip U251 cells HCMV Protein expression 
SELDI技术分析体外培养不同肝细胞株差异蛋白的表达被引量:1
《第四军医大学学报》2006年第16期1441-1444,共4页丁守怡 钱冬萌 牟文凤 闫志勇 宋旭霞 王斌 
国家自然科学基金(30471527)与HCMV感染相关的婴儿肝炎综合征蛋白标志物的研究;青岛科技局(05-1-NS-70)病毒性肝炎;肝硬化;肝癌的差异蛋白质组学研究及早期诊断蛋白质谱的筛选
目的:运用表面增强激光解吸/离子似飞行时间质谱(SELDI-TOF-MS)技术,检测体外培养的肝癌细胞株(HepG2),转染乙肝病毒的肝癌细胞株(HepG2.2.15)与正常肝细胞株(LO2)蛋白质的差异表达,筛选肝细胞癌的标志蛋白,为进一步研...
关键词:表面增强激光解吸/离子化/飞行时间质谱 蛋白质陈列分析 肝肿瘤 肿瘤细胞 培养的 差异蛋白 
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