Project supported by the National Natural Science Foundation of China (Nos. 39880003, 20175016).
Based on the monodisperse poly(glycidyl methacrylate-co-ethylenedimethacrylate) beads (PGMA/EDMA) with macropore as a medium, a new hydrophilic medium cation exchange (MCX) stationary phase for HPLC was synthesi...
This work is supported by the National Natural Science Foundation of china (No. 20175016 and No. 20475042) ;the Foundation of Key Laboratory of Modem Separation Science in Shaanxi Province (No. 05JS61).
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) in inclusion bodies was solubilized by 8 mol/L urea solution and subsequently precipitated by acetone to improve its purity. After that, the precipit...
supported by the National Natural Science Foundation of China(No.39880003 and 20175016).
The addition of packing material for high performance hydrophobic interaction chromatograghy (HPHIC) into the denaturant solution to prevent, or depress protein aggregation in the denatuaration process is presented....