Project supported by the State Key Program of Basic Research of China(No.2002CB512802);the National Natural Science Foundation of China(No.20372069,29725203,and 20072042);a Shanghai Science and Technology Commission Grant No 02DJ14006);the Key Project for New Drug Research from the Chinese Academy of Sciences;the National High Technology Research and Development Program of China(No 2002AA233061,2002AA104270,2002AA233011,and 2003AA235030);supported by the Foundation of East China University of Science and Technology for Research(No.YC0142101).
Aim: To investigate the dynamic properties of protein-tyrosine phosphatase (PTP) IB and reveal the structural factors responsible for the high inhibitory potency and selectivity of the inhibitor SNA for PTPIB. Meth...